当前位置: 首页 > 文章 > 霍寿黑猪和长白猪TLR4基因第3外显子的SNP检测及其遗传差异分析 安徽农业大学学报 2014,41 (5) 98-102
Position: Home > Articles > Single nucleotide polymorphism(SNP) detection and genetic difference analysis of TLR4 gene exon 3 in Huoshou black and landrance pigs Journal of Anhui Agricultural University 2014,41 (5) 98-102

霍寿黑猪和长白猪TLR4基因第3外显子的SNP检测及其遗传差异分析

作  者:
朱卫华;杜恒;杨勇;薛玮玮;孟云;黄龙;张晓东;丁月云;殷宗俊
单  位:
安徽安泰农业集团;安徽农业大学动物科技学院
关键词:
霍寿黑猪;长白猪;TLR4基因;SNP;群体遗传学分析
摘  要:
为了对安徽地方猪霍寿黑猪和引进猪种长白猪TLR4基因进行多态性分析,采用PCR-SSCP结合PCR产物双向测序的方法研究了86头霍寿黑猪及44头长白猪2个群体共130个样本TLR4基因外显子3部分片段的单核苷核多态性,并对检测到的突变位点进行群体遗传学分析。结果检测到1个突变C1027A,C1027A突变为错义突变。群体遗传学分析表明,该多态位点基因型的分布在霍寿黑猪和长白猪2个中外猪种间存在极显著差异(P<0.01)。C1027A位点多态性在地方猪品种和引进品种间的差异,是否与品种抗病性有关,值得进一步研究。
译  名:
Single nucleotide polymorphism(SNP) detection and genetic difference analysis of TLR4 gene exon 3 in Huoshou black and landrance pigs
作  者:
ZHU Weihua;DU Heng;YANG Yong;XUE Weiwei;MENG Yun;HUANG Long;ZHANG Xiaodong;DING Yueyun;YIN Zongjun;Anhui Provincial Key Laboratory of Local Livestock and Poultry Genetic Resources Conservation and Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University;Anhui Antai Agriculture Group;
关键词:
Huoshou black pig;;Landrance;;TLR4;;SNP;;population genetics analysis
摘  要:
Single nucleotide polymorphisms of TLR4 exon 3 in 86 Huoshou black and 44 landrance pigs(a total of 130 individuals in two populations) were detected using PCR-SSCP and bi-directional DNA sequencing. The detected SNPs were further analyzed using population genetics for their genetic variations. One SNP site(C1027A) was detected to be a no-synonymous SNP site. The result showed that the distribution of the C1027A-polymorphic genotype was significantly different(P<0.01) at the 1027 locus between Landrace and Huoshou black pigs. Such differences at the 1027 locus between local and introduced pigs might correlate with resistance of certain diseases.

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