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当前位置: 首页 > 文章 > 棉花雄性不育“三系”过氧化物酶同工酶等电聚焦电泳分析 作物学报 1991,17 (5) 376-380
Position: Home > Articles > Isoelectrofocusing Analysis on Peroxidase Isozymes of Male Sterile“Three Lines”of Cotton Acta Agronomica Sinica 1991,17 (5) 376-380

棉花雄性不育“三系”过氧化物酶同工酶等电聚焦电泳分析

作  者：
邱竟;张久绪
单  位：
中国农业科学院棉花研究所
关键词：
过氧化物酶同工酶;等电聚焦;不育系;保持系;恢复系
摘  要：
通过等电聚焦电泳,在 pH4—6区间显示出棉花质核雄性不育系、保持系、恢复系在子叶、真叶、茎、花药等部位中共有20种不同的过氧化物酶同工酶带,其中有4条为这4种部位所共有。同时,叶片、茎、花药都各有其专一酶带。供试的“三系”在同一部位中均具有一些相同的基本酶带,相同系间差异较小,表现出它们有着某些基本一致的遗传背景。受育性基因的影响,不育系(A 系)与可育系(B 系,R 系)之间有着明显差别,在子叶、真叶、茎中 A 系的过氧化物酶同工酶多于 B 系和 R 系。这可能表明雄性不育基因在幼苗期已经开始调控过氧化物酶同工酶的合成。进入“小孢子单核期”的 A 系花粉粒已发生败育,其花药的过氧化物酶同工酶明显少于 B 系和 R 系。
译  名：
Isoelectrofocusing Analysis on Peroxidase Isozymes of Male Sterile“Three Lines”of Cotton
作  者：
Qiu Jing Zhang Jiuxu (Cotton Research Institute of CAAS,Anyang,Henan,455112)
关键词：
Peroxidase Isozymes;;lsoelectrofocusing;;A-line;;B-line;;R-line
摘  要：
The isolectrofocusing analysis showed that there were 20 bands of peroxidaseisozymes at pH 4—6 for cotyledons,true leaves,stems and anthers of threeliens(A-line,B-line and R-line),among these bands,4 ones were responsible forall 4 tissues.In addition,leaves,stems and anthers also had thier special bands,respectively.The three lines all showed some of similar bands in the same tissue partwith less differences between alike lines,indicating that they had some similar geneticbackground.There were obvious differences between sterile A-line and fertile B-lineand R-line due to the influence exerted by male sterile genes.The peroxidaseisozymes in cotyledons,true leaves and stems of A-line were more than that ofB-line and R-line,which showed significance for research and identification of threelines of cotton and showed that the male sterile genes controled the synthesis ofperoxidase isozymes in seedling stage.The abortive pollens of A-line in “microsporemonocaryon” period occurred,in which the quantity of peroxidase isozymes in antherswere obvious less than that of B-line and R-line.

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