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当前位置: 首页 > 文章 > 响应面法优化微波提取枳椇子多糖及其清除羟自由基活性研究 食品科学 2013,34 (12) 56-60
Position: Home > Articles > Optimization by Response Surface Analysis of Microwave-Assisted Extraction and Hydroxyl Radical Scavenging Activity of Polysaccharide from Hovenia acerba Lindl FOOD SCIENCE 2013,34 (12) 56-60

响应面法优化微波提取枳椇子多糖及其清除羟自由基活性研究

作  者：
冯自立;党娅
单  位：
陕西理工学院陕西省资源生物重点实验室
关键词：
枳椇子;多糖;微波提取;响应曲面;清除.OH活性
摘  要：
目的:建立微波辅助提取枳椇子多糖优化工艺模型,初探其清除羟自由基(.OH)活性能力。方法:在单因素试验基础上,以枳椇子多糖提取率为指标,采用响应曲面统计法的Box-Behnken模型对影响微波提取工艺关键因素:微波功率、液固比、提取时间进行优化探讨。通过.OH与水杨酸反应机理测定枳椇子多糖体外抗氧化活性。结果:所得优化工艺条件为微波功率504.3W、液固比25:1、提取时间21.9min,该条件下多糖提取率为10.84%;枳椇子多糖对.OH的IC50为205.87μg/mL,清除能力相对较强。
译  名：
Optimization by Response Surface Analysis of Microwave-Assisted Extraction and Hydroxyl Radical Scavenging Activity of Polysaccharide from Hovenia acerba Lindl
作  者：
FENG Zi-li,DANG Ya(Shaanxi Provincal Key Laboratory of Bio-resources,Shaanxi University of Technology,Hanzhong 723001,China)
关键词：
Hovenia acerba Lind;polysaccharide;microwave-assisted extraction;response surface methodology;free radical scavenging activity
摘  要：
Objective: To establish optimal conditions for the microwave-assisted extraction of ploysaccharide from Hovenia acerba Lindl by means of mathematical modeling and to explore the hydroxyl radical scavenging activity of the polysaccharide extract from Hovenia acerba Lindl.Methods: Box-Behnken design was used to develop a regression model indicating the effect of three key process parameters including microwave power,solvent-to-solid ratio and extractiom time on the extraction efficiency of polysaccharide.Based on the model,these extraction prameters were optimized by response surface analysis.The antioxidant activity in vitro of the polysaccharide obtained was evaluated based on the reaction between hydroxyl radical and salicylic acid.Results: The optimal extraction conditions were obtained as follows: 504.3 W,25:1 and 21.9 min for microwave power,solvent-to-solid ratio and extraction time,respectively.The extraction efficiency of polysaccharide under these conditions was 10.84%.The resulting polysaccharide had strong hydroxyl radical scavenging activity with an IC50of 205.87 μg/mL.

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