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Position: Home > Articles > Several Problems Worthy of Being Noticed During Agarose Gel Electrophoresis Guizhou Agricultural Sciences 2004,32 (6) 31-32

琼脂糖凝胶电泳操作中值得注意的几个问题

作  者:
朱英;陶刚;刘作易;宋吉轩
单  位:
贵州省农业生物技术重点实验室
关键词:
琼脂糖;凝胶电泳;DNA
摘  要:
针对琼脂糖凝胶电泳中经常出现的电泳图谱条带模糊、弯曲 ,图谱背景荧光较强 ,凝胶胶面不平以及重复性差等问题 ,提出了相应的简便可行的解决方法
译  名:
Several Problems Worthy of Being Noticed During Agarose Gel Electrophoresis
作  者:
ZHU Ying~(1,2), TAO Gang~(1,3), LIU Zuo-yi~(1,4), SONG Ji-xuan~(1,2)(1. The Key Laboratory for Agri. Biotechnology of Guizhou, Guiyang 550006; 2. Institute of Biotechnology, Guizhou Academy of Agri. Sci., Guiyang 550006; 3. Institute of Plant Protection, Guizhou Academy of Agri. Sci., Guiyang 550006; 4. Guizhou Academy of Agri. Sci., Guiyang 550006, China)
关键词:
agarose; gel electrophoresis; DNA
摘  要:
In general, those problems such as the dim and bending band, strong fluorescence background, bad repetition often occured during DNA agarose gel electrophoresis. They were solved by using some simple and effective methods expressed in this (paper).

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