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Position: Home > Articles > EDTA-Na_2 Extraction of Agarose with Response Surface Optimization Fujian Journal of Agricultural Sciences 2013,28 (2) 158-165

响应面优化琼脂糖的EDTA-Na_2法提取工艺

作  者:
李龙;苏永昌;吴成业
单  位:
福建省水产研究所;福建农林大学食品科学学院
关键词:
响应面法;EDTA-Na2法;琼脂糖
摘  要:
探讨了提取工艺中主要影响因子EDTA-Na2浓度、提取温度、提取时间对硫酸根含量及凝胶强度的影响,并利用响应面法优化提取工艺,得出最佳工艺参数为:EDTA-Na2浓度4.5g.L-1,提取温度65℃,提取时间3h,在最佳提取工艺的条件下制得的琼脂糖,其硫酸根含量为0.48%,凝胶强度为1 073.09g.cm-2。对最优工艺参数进行重复性验证,相对误差在1.0%以内。表明该方法具有较高的准确性和重复性,可作为琼脂糖的粗提方法。
译  名:
EDTA-Na_2 Extraction of Agarose with Response Surface Optimization
作  者:
LI Long1,2,SU Yong-chang2,WU Cheng-ye2(1.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China; 2.Fisheries Research Institute of Fujian,Xiamen,Fujian 361013,China)
关键词:
Response surface method;EDTA-Na2 method;agarose
摘  要:
This paper studied the technique of agarose extraction with EDTA-Na2 method.The main factors discussed were: EDTA-Na2 concentration,extraction temperature and extraction time on influencing in sulfate radical content and gel strength.Using the response surface method to optimize the extraction process,the best parameters were EDTA-Na2 concentration 4.5 g·L-1,extraction temperature 65℃ and extracting time 3 h.With the optimized parameters,the sulfate radical content and gel strength of agarose extracted were 0.48% and 1 073.09 g·cm-2,respectively.The optimal extraction parameters were verified by repetitive tests with relative error <1.0%.It is suggested that EDTA-Na2 method is accurate and repeatable in using for agarose extraction.

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