当前位置: 首页 > 文章 > 新型鸭病毒性肝炎病毒RT-PCR检测方法的建立 中国家禽 2009,31 (18) 19-21
Position: Home > Articles > Establishment of Reverse Transcriptional Polymerase Chain Reaction for Detection of New Type Duck Hepatitis Virus China Poultry 2009,31 (18) 19-21

新型鸭病毒性肝炎病毒RT-PCR检测方法的建立

作  者:
宋永峰;温纳相;宋延华;周全;周丽;高恒
单  位:
广东温氏食品集团研究院
关键词:
新型鸭肝炎病毒;RT-PCR;检测方法
摘  要:
根据GenBank中已发表的1型鸭病毒性肝炎(duck hepatitis virus type1,DHV-1)和新型鸭病毒性肝炎(new type duck hepatitis virus,N-DHV)全基因序列,在非同源区域设计一对新型鸭病毒性肝炎特异性引物P1/P2,对鸭病毒性肝炎病毒、鸭瘟病毒、鹅细小病毒细胞毒、流感病毒等鸭常见病病原进行RT-PCR扩增。结果表明引物特异性良好,引物P1/P2仅能特异性扩增出N-DHV的638bp基因片段;敏感性试验结果表明:N-DHV RNA最低检出量为21pg。同时对临床样品的检测中发现DHV-1和N-DHV存在混合感染的情况。该方法的建立为鸭病毒性肝炎病毒尤其是新型鸭病毒性肝炎病毒的鉴定及快速诊断提供了可靠的依据。
译  名:
Establishment of Reverse Transcriptional Polymerase Chain Reaction for Detection of New Type Duck Hepatitis Virus
作  者:
SONG Yongfeng,WEN Naxiang,SONG Yanhua,ZHOU Quan,ZHOU Li,GAO Heng (Research Institute of Guangdong Wen′s Foodstuffs Group Company,Xinxing,Guangdong 527400)
关键词:
new type duck hepatitis virus;RT-PCR;detecting method
摘  要:
Based on the published complete sequences of duck hepatitis virus type 1 (DHV-1)and new type duck hepatitis virus (N-DHV),one pair of specific primer P1/P2 was designed and used to amplify by PCR or RT-PCR to nucleic acids of DHV,duck plauge virus,goose plauge virus,avian influenza virus.The results indicated that 638 bp specific band could be amplified by primer P1/P2 only from N-DHV RNA and no specific bands of the same sizes were amplified from other viruses. As little as 21 pg of N-DHV RNA could be detected in this RT-PCR. Meanwhile,mixed infection of DHV-1 and N-DHV were been in detection of samples. This method was significant for identification and diagnosis of DHV,especially N-DHV.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊