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Position: Home > Articles > Selection of a Vaccine Candidate Strain of Novel Duck Hepatitis Virus China Animal Health Inspection 2010,27 (1) 41-43

新型鸭肝炎病毒疫苗候选株的筛选

作  者：
刘文华;李宇琴;罗飞;刘相娥;杜元钊;尹景超;党启峰
单  位：
扬州大学兽医学院;青岛农业大学动物科技学院;青岛易邦生物工程有限公司
关键词：
新型鸭肝炎病毒;RT-PCR;血清中和试验;鸭胚肝细胞培养
摘  要：
本试验对临床患病鸭分离的一株疑似鸭肝炎病毒进行了血清型鉴定和毒力测定。利用Ⅰ型和新型鸭肝炎病毒鉴别引物对提取的病毒RNA进行RT-PCR扩增;将分离病毒分别与Ⅰ型和新型鸭肝炎病毒阳性血清进行中和试验;根据测定的病毒ELD50,进行雏鸭攻毒和鸭胚肝细胞接毒试验。结果表明:RT-PCR扩增出了与新型鸭肝炎病毒预期片段相符的705bp条带;分离病毒不能被Ⅰ型鸭肝炎病毒阳性血清中和,新型鸭肝炎病毒阳性血清对该病毒的中和效价是1:200,说明该分离株属于新型鸭肝炎病毒。该毒株的ELD50为10-5.7/0.2mL,能引起攻毒鸭与临床病例一致的症状和病变以及显著的肝细胞病变,可作为新型鸭肝炎病毒的疫苗候选株开发应用。
译  名：
Selection of a Vaccine Candidate Strain of Novel Duck Hepatitis Virus
作  者：
Liu Wen-hua1,Li Yu-qin2,3,Luo Fei2,Liu Xiang-e3,Du Yuan-zhao3,Ying Jing-chao3,Dang Qi-feng3 (1.College of Animal Science & Technology, Qingdao Agricultural University ,Qingdao,266109; 2.College of Veterinary Medicine, Yangzhou University, Yangzhou,225009;3.Yebio Bioengineering Corporation, Qingdao,266032)
关键词：
duck hepatitis virus (DHV); RT-PCR; serum neutralization test; duck embryo liver cell culture
摘  要：
Serotype and toxicity of a suspected duck hepatitis virus strain isolated from clinical dead duck with duck virus hepatitis symptoms were tested. RT-PCR method was established to identify the virus with type 1 and novel DHV primers respectively. Neutralization test was carried out with type 1 and novel DHV positive serum. Furthermore, duckling challenge and duck embryo liver cell inoculation were done with the virus according to the ELD50. Results indicated that expected fragment about 705bp of novel DHV was amplified by RT-PCR, and ELD50 of the virus was 10-5.7/0.2mL. The isolated virus could not be neutralized by type 1 DHV positive serum, while neutralizing titer of novel DHV positive serum to the isolated virus was about 1:200. Typical symptoms and CPE could also be observed obviously when the isolated virus was inoculated to ducklings and duck embryo liver cell. All the results showed that the identified novel DHV could be developed and applied as a vaccine candidate strain.

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