中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 口灌醋酸棉酚对鲤血清转氨酶、肝脏抗氧化酶活力及肝细胞凋亡的影响 河南农业科学 2019 (7) 128-135
Position: Home > Articles > 口灌醋酸棉酚对鲤血清转氨酶、肝脏抗氧化酶活力及肝细胞凋亡的影响 Journal of Henan Agricultural Sciences 2019 (7) 128-135

口灌醋酸棉酚对鲤血清转氨酶、肝脏抗氧化酶活力及肝细胞凋亡的影响

作  者：
张孟丹;梁俊平;张静;许振山;李慧;张建新;聂国兴
单  位：
河南师范大学水产学院
关键词：
鲤;醋酸棉酚;肝脏抗氧化酶;血清转氨酶;活力;细胞凋亡
摘  要：
为了评价醋酸棉酚对鲤肝脏的急性毒性效应,对鲤单次口灌20 mg/kg醋酸棉酚,分别于口灌后0.5、1、2、4、6、12、24、48、72 h时测定鲤血清中谷草转氨酶(GOT)、谷丙转氨酶(GPT)和肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力;观察口灌醋酸棉酚24 h时,鲤肝细胞的组织学特征和凋亡现象,并检测鲤肝脏中caspase3、caspase6、caspase7基因表达水平.结果显示,对鲤单次口灌20 mg/kg醋酸棉酚后,其血清GOT活力在0.5 h时显著高于对照组未口灌醋酸棉酚的鲤(P<0.05),在48、72 h时显著低于对照组(P<0.05),在1、2、4、6、12、24 h时与对照组相比无显著性差异(P>0.05);其血清GPT活力在0.5、1 h时显著高于对照组(P<0.05),4、48 h时显著低于对照组(P<0.05),2、6、12、24、72 h时与对照组相比无显著性差异(P>0.05).鲤肝脏SOD活力在0.5、1、2、48 h时显著高于对照组(P<0.05),4、12 h时显著低于对照组(P<0.05),6、24、72 h时与对照组相比无显著性差异(P>0.05);GSH-Px活力在0.5、1、2、24、48、72 h时显著高于对照组(P<0.05),6、12 h时显著低于对照组(P<0.05),4 h时与对照组相比无显著性差异(P>0.05).口灌醋酸棉酚24 h时,鲤肝细胞体积和细胞核大小无明显变化,细胞未发生凋亡现象;鲤肝脏中caspase3、caspase6、caspase7基因表达量均显著低于对照组(P<0.05).综上,对鲤单次口灌20 mg/kg醋酸棉酚后,鲤的肝功能短时间内受到了一定程度影响,但未引起组织病变或细胞凋亡,可能是通过激活机体抗氧化酶系统缓解了醋酸棉酚的毒性作用.
作  者：
ZHANG Mengdan;LIANG Junping;ZHANG Jing;XU Zhenshan;LI Hui;ZHANG Jianxin;NIE Guoxing;College of Fisheries,Henan Normal University;
单  位：
ZHANG Mengdan%LIANG Junping%ZHANG Jing%XU Zhenshan%LI Hui%ZHANG Jianxin%NIE Guoxing%College of Fisheries,Henan Normal University
关键词：
Cyprinus carpio;;Gossypol acetate;;Liver antioxidant enzyme;;Serum aminotransferase;;Enzyme activity;;Apoptosis
摘  要：
In order to evaluate the acute hepatotoxicity of gossypol acetate in Cyprinus carpio, the activities of serum gluamic-oxalacetic transaminase(GOT),glutamic pyruvic transaminase(GPT), hepatic superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) were analyzed at 0.5,1,2,4,6,12,24,48,72 h after oral administration of gossypol acetate at 20 mg/kg.The histological characteristics of liver, TUNEL of hepatocytes, and the genes expression of caspase3, casspase6, caspase7 were analyzed at 24 h after oral administration.The results showed that the serum GOT activity of 20 mg/kg dose group was significantly higher than the control group at 0.5 h(P<0.05),and it was significantly lower at 48 h and 72 h than the control group(P<0.05),and there was no significant difference compared with the control group at 1,2,4,6,12,24 h(P>0.05).The serum GPT activity was significantly higher than the control group at 0.5 h and 1 h(P<0.05),it was lower than the control group at 4 h and 48 h(P<0.05),and there was no significant difference compared with the control group at 2,6,12,24,72 h(P>0.05).The activity of SOD in liver was significantly higher than the control group at 0.5,1,2,48 h(P<0.05),significantly lower at 4 h and 12 h than the control group(P<0.05),and there was no significant difference compared with the control group at 6,24,72 h(P>0.05).The GSH-Px activity in liver was significantly higher than that of the control group at 0.5,1,2,24,48,72 h(P<0.05),and significantly lower than the control group at 6 h and 12 h(P<0.05), and there was no significant difference between the gossypol group and the control group at 4 h(P>0.05).There was no significant change in the volume of hepatocytes and nucleus,and there was no apoptosis in the cells of Cyprinus carpio at 24 h after oral administration.The expression levels of caspase3,caspase6 and caspase7 in liver were significantly lower than those in the control group(P<0.05).In summary,the results suggested that the liver function was affected in a short time following oral administration,but the liver was not damaged, and the free gossypol did not induce apoptosis in liver cells of Cyprinus carpio.Cyprinus carpio may increase its own antioxidant enzyme activities to reduce the toxic effects of gossypol acetate.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46