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Position: Home > Articles > Analysis of the Antigenic Epitopes Recognized by Monoclonal Antibody against Swine hepatitis E virus Progress in Veterinary Medicine 2008,29 (4) 46-48

猪戊型肝炎病毒单克隆抗体的抗原识别位点分析

作  者:
李丹丹;张馨心;赵立平;相文华
单  位:
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;东北农业大学动物医学院
关键词:
猪戊型肝炎病毒;单克隆抗体;抗原位点
摘  要:
采用ELISA叠加试验,对6株具有ELISA反应特性的猪戊型肝炎病毒(SHEV)单克隆抗体(McAb-αC11、αC12、γH1、γF8、BC4和CH8)的抗原识别位点进行检测。抗体反应增值结果表明,6株单抗分别针对4个不同抗原位点,McAb-γH1,BC4,CH8识别的位点各不相同,其中McAb-γH1、BC4识别的位点有部分重叠,McAb-αC11、αC12,γF8识别同一位点,位于γH1,BC4识别位点的重叠区内。
译  名:
Analysis of the Antigenic Epitopes Recognized by Monoclonal Antibody against Swine hepatitis E virus
作  者:
LI Dan-dan1,2,ZHANG Xin-xin2,ZHAO Li-ping1,XIANG Wen-hua1(1.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang,150001,China;2.Veterinary Medicine College,Northeast Agriculture University,Harbin,Heilongjiang,150030,China)
关键词:
Swine hepatitis E virus;McAb;epitope
摘  要:
Antigenic epitopes recognized by six strains of monoclonal antibodies(McAb-αC11、αC12 、γH1 、γF8 、BC4、CH8) with ELISA reaction characteristics against swine hepatitis E virus(SHEV)were detected by means of competitive binding assay.The result indicated that the six strains of McAbs were specific for different kinds of epitopes on SHEV.Among them,McAb-γH1,McAb-BC4 and McAb-CH8 recognized different epitopes,respectively.The epitope recognized by McAb-γH1 overlapped partly that of McA b-BC4.McAb-αC11 and McAb-αC12 were identical to McA b-γF8.The epitope of them was supposed to be located in the overlapping region between epitope-γH1 and epitope-BC4.

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