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Position: Home > Articles > Extraction and Analysis of Phospholipids in Egg Yolk FOOD SCIENCE 2007,28 (7) 373-376

鸡蛋黄中磷脂的提取及分析

作  者:
张佑红;陈燕;朱雄伟;秦琴;吴元欣;杜进;谈雅静;候畅;赵亮;吕中
单  位:
北京大学医学部;武汉化工学院;武汉工程大学湖北省新型反应器与绿色化学工艺重点实验室
关键词:
蛋黄磷脂;提取;纸层析;薄层层析
摘  要:
本研究利用磷脂在丙酮中的相对不溶性,将磷脂从蛋黄中提取出来,用分光光法在630nm下测量蛋黄中磷脂的含量。然后以纸层析为主要的定性检测手段来检测磷脂X基团,用薄层色谱法对磷脂混合物的各个成分进行定性分析。
译  名:
Extraction and Analysis of Phospholipids in Egg Yolk
作  者:
ZHANG You-hong1,CHEN Yan1,ZHU Xiong-wei1,QIN Qin1,WU Yuan-xin1,DU Jin2, TAN Ya-jing3,HOU Chang3,ZHAO Liang1,LU Zhong1 (1.Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China;2.Medicine School, Beijing University, Beijing 100083, China; 3.Wuhan Institute of Technology, wuhan 430023, China)
关键词:
lecithin;extract;paper chromatography;thin layer-chromatography
摘  要:
Based on relative indissolubility of phospholipid in acetone,phospholipids in egg yolk were extracted in this research. The content of phosphorus was determined with spectrophometry at 630nm. Group X in phospholipid was detected by paper chromatography. At least four fractions of the phospholipids were separated by thin layer chromagraphy and the phenomena was explained.

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