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Position: Home > Articles > Purification of Glycyrrhizic Acid from Glycyrrhiza Using Macroporous Resin FOOD SCIENCE 2013,34 (6) 93-97

高效液相色谱法评价大孔树脂纯化甘草酸工艺

作  者:
孙啸涛;李柰;王昌涛
单  位:
北京工商大学北京市植物资源研究开发重点实验室
关键词:
甘草酸;大孔树脂;高效液相色谱;紫外分光光度法
摘  要:
对甘草纯化甘草酸的工艺进行系统研究。通过对DA201-C和AB-8两种型号大孔树脂静态吸附和解吸的比较,选择AB-8型大孔树脂对甘草酸进行纯化;静态吸附解吸实验表明,AB-8型大孔树脂纯化甘草酸的最佳工艺条件为上样液pH6.0、上样液质量浓度5.89mg/mL、洗脱液50%乙醇溶液;应用高效液相色谱法检测甘草酸纯度的方法,结果显示,经AB-8型大孔树脂纯化后的甘草酸纯度52.3%,重结晶后纯度为76.0%。
译  名:
Purification of Glycyrrhizic Acid from Glycyrrhiza Using Macroporous Resin
作  者:
SUN Xiao-tao,LI Nai,WANG Chang-tao*(Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China)
关键词:
glycyrrhizic acid;macroporous resin;high performance liquid chromatography(HPLC);ultraviolet spectrophotometry
摘  要:
The purification of glycyrrhizic acid from glycyrrhiza using macroporous resin was study.Static absorption and desorption experiments were carried out to screen the optimal resin from macroporous resins DA201-C and AB-8.AB-8 was found to have better performance than DA201-C.In static adsorption and desorption experiments,the optimal purification conditions of glycyrrhizic acid by resin AB-8 were sample pH 6.0,5.89 mg/mL sample concentration,and 50% ethanol as the desorption solvent.In addition,the purity of purified glycyrrhizic acid was determined by HPLC to be 52.3%,and was increased to 76.0% after recrystallization.

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