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当前位置: 首页 > 文章 > 大豆疫霉菌(Phytophthora sojae)抗甲霜灵菌株筛选及生物学特性 中国油料作物学报 2014,36 (5) 623-629
Position: Home > Articles > Screening and biological characteristics of metalaxyl-resistant isolates of Phytophthora sojae Chinese Journal of Oil Crop Sciences 2014,36 (5) 623-629

大豆疫霉菌(Phytophthora sojae)抗甲霜灵菌株筛选及生物学特性

作  者：
国莉玲;左豫虎;柯希望;王晓杰;韩德俊;韩青梅;康振生;黄丽丽
单  位：
旱区作物逆境生物学国家重点实验室/西北农林科技大学;黑龙江八一农垦大学农学院
关键词：
大豆疫霉菌;甲霜灵;抗性诱导;药剂驯化;抗药稳定性;生物学特性
摘  要：
以大豆疫霉野生菌株Pmg1为亲本,采用药剂驯化法获得抗甲霜灵菌株。初步研究抗药菌株的抗性水平、菌丝生长阶段抗性的稳定性、菌落生长速率、菌落形态、游动孢子囊和卵孢子产生能力等生物学特性,并利用光学显微镜观察其菌丝、游动孢子囊和卵孢子的形态。结果表明,获得5株抗甲霜灵菌株Pmg1-Mtr1、Pmg1-Mtr2、Pmg1-Mtr3、Pmg1-Mtr4、Pmg1-Mtr5,抗性水平分别可达到野生亲本的3 793.7、5 581.7、5 773.9、4 409.3和5 476.0倍,且抗性可在菌丝阶段稳定遗传。与野生菌株Pmg1相比,抗药菌株的菌落生长速率明显降低;菌丝、游动孢子囊和卵孢子在形态上与野生菌株相比没有明显变化,但抗药菌株游动孢子囊和卵孢子的产量显著降低。表明大豆疫霉菌对甲霜灵容易产生抗性,抗药菌株在菌丝阶段抗药性的保持对甲霜灵没有依赖性。菌株除抗药性提高、游动孢子囊和卵孢子产生能力下降外,其它生物学性状没有发生显著变化。
译  名：
Screening and biological characteristics of metalaxyl-resistant isolates of Phytophthora sojae
作  者：
GUO Li-ling;ZUO Yu-hu;KE Xi-wang;WANG Xiao-jie;HAN De-jun;HAN Qing-mei;KANG Zhen-sheng;HUANG Li-li;College of Agronomy,Heilongjiang Bayi Agricultural University;State Key Laboratory of Crop Stress Biology for Arid Areas /Northwest A&F University;
关键词：
Phytophthora sojae;;Metalaxyl;;Inducible resistance;;Chemical taming;;Stability of resistance;;Bi-ological characteristics
摘  要：
To understand the characteristics of metalaxyl- resistant strain of Phytophthora sojae,metalaxyl-resistant( Mtr) isolates were obtained by chemical taming from wild- type parent isolate Pmg1. The investigated biological characteristics included resistance level,stability of resistance in the growth of hyphae,colony growth rate and morphology,production capability of zoosporangium and oospores. Morphology of hyphae,zoosporangium and oospores were also observed under optical microscope. Results showed that the resistance levels of Mtrisolates were 3 793. 7,5 581. 7,5 773. 9,4 409. 3 and 5 476. 0 folds of wild- type parent respectively,and the resistance were stably inherited during hyphae growth stage. Compared with wild- type parent Pmg1,Mtrisolates colony growth reduced. The morphology of hyphae,zoosporangium and oospores had no significant differences from those of wild- type parent,but zoosporangium and oospores production decreased significantly. The result indicated that it was easy for P. sojae to produce resistance to metalaxyl,and the resistance of Mtrisolates could remain in hyphae growth stage without metalaxyl. The resistance of Mtrisolates was increased and the production capability of zoosporangium and oospores was decreased,other biological characteristics had no significant change.

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