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Position: Home > Articles > Rapid Determination of Rhodamine B Residues in Fruit Drink by Fluorescent Probe Assay FOOD SCIENCE 2012,33 (18) 258-260

荧光探针法快速检测果汁中罗丹明B残留量

作  者:
梁宇;黄世江;王超海;程定玺
单  位:
河南师范大学化学与环境科学学院新乡学院化学与化工学院;河南师范大学化学与环境科学学院;新乡学院化学与化工学院;河南科技学院新科学院
关键词:
荧光探针;伊红Y;罗丹明B;残留
摘  要:
建立以伊红Y为荧光探针测定罗丹明B残留量的荧光光谱法。在pH6.8的Sфrensen缓冲溶液中,一定浓度的阳离子表面活性剂溴化十六烷基三甲基铵(CTMAB)能使阴离子染料伊红Y的荧光强度显著增强,当加入一定量的罗丹明B后其荧光发生猝灭,且猝灭程度在一定范围内与加入的罗丹明B浓度呈正比。通过改变单因素,优化实验条件,获得的线性范围为0~0.9mg/L,检出限为0.0016mg/L,回收率为101.3%~113.7%,相对标准偏差为2.76%~4.98%。该方法具有简便快捷、选择性好、灵敏度高等优点,适用于果汁饮料制品中罗丹明B的检测。
译  名:
Rapid Determination of Rhodamine B Residues in Fruit Drink by Fluorescent Probe Assay
作  者:
LIANG Yu1,2,HUANG Shi-jiang1,WANG Chao-hai2,CHENG Ding-xi1,(1.School of Chemistry and Environmental Science,Henan Normal University,Xinxiang 453007,China;2.College of Chemistry and Chemical Engineering,Xinxiang University,Xinxiang 453003,China)
关键词:
fluorescent probe;eosin Y;rhodamine B;residue
摘  要:
A new fluorescence spectrometry method for the determination of rhodamine B(RhB) was established by the use of eosin Y(EY) as fluorescent probe.In Sфrensen buffer solution at pH 6.8,the fluorescence intensity of anionic dye EY can be significantly enhanced by adding cetyltrimethylammonium bromide(CTMAB),a cationic surfactant.However,RhB has the ability to quench the fluorescence in a proportional manner within a certain concentration range.In this study,one-factor-at-a-time designs were used to determine the optimal experimental conditions for the determination of RhB.The linear range,detection limit and average recovery rate of the developed assay were 0-0.9 mg/L,0.0016 mg/L,and 101.3%-113.7% with relative standard deviation of 2.76%-4.98%,respectively.This method is simple,quick,selective and sensitive so that it is suitable for the determination of RhB residues in fruit drink products.

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