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当前位置: 首页 > 文章 > 荧光探针法研究铅离子对小牛胸腺DNA构象的影响 安徽农业科学 2009,37 (34) 125-126+132
Position: Home > Articles > Research on the Influences of Lead Ions on the Conformation of Calf-thymus DNA by Fluorescence Probe Method Journal of Anhui Agricultural Sciences 2009,37 (34) 125-126+132

荧光探针法研究铅离子对小牛胸腺DNA构象的影响

作  者：
赵霞;范阔海
单  位：
山西农业大学临床兽医实验室
关键词：
荧光探针;小牛胸腺DNA;DNA构象
摘  要：
[目的]利用荧光探针法研究铅离子与DNA的作用方式。[方法]采用紫外可见分光光度法检测了DNA的纯度,采用荧光滴定法研究了铅离子对DNA构象的影响。[结果]随着铅离子的加入,DNA-EB复合物的荧光强度逐渐降低。在DNA-EB体系中加入铅离子后,荧光强度随着铅离子浓度的增加而降低,且荧光峰位略有红移。按生物分子的荧光寿命τ0为10ns计算,DNA-EB与铅离子的双分子猝灭常数分别为1.01×1013和4.0×1014L/(mol.s),二者均大于生物分子的最大动态猝灭常数2.0×1010L/(mol.s),说明由铅离子引起的DNA-EB的荧光猝灭属于静态猝灭。根据斜率和外推截距计算出DNA-EB与猝灭剂的解离常数为1.13×10-5mol/L。铅离子与DNA-EB的结合位点为5.56,结合常数为1.51×1029。[结论]铅离子容易与DNA结合,引起DNA构象的变化。
译  名：
Research on the Influences of Lead Ions on the Conformation of Calf-thymus DNA by Fluorescence Probe Method
作  者：
ZHAO Xia et al(Laboratory of Clinical Veterinary,Shanxi Agricultural University,Taigu,Shanxi 030801)
关键词：
Fluorescence probe;Calf-thymus DNA;DNA conformation
摘  要：
[Objective] The purpose was to research the action mode of lead ions and DNA by fluorescent probe method.[Method] The purity of DNA was detected by UV-vis spectrophotometry and the influence of lead ions on DNA conformation was researched by fluorescence titration.[Result] The fluorescence intensity of DNA-EB compound was decreased gradually along with the addition of lead ions.After the lead ions were added into DNA-EB system,the fluorescence intensity was decreased as the concn.of lead ions was increased and the fluorescent peak-position was a little red shifted.The fluorescence lifetime τ0 of biological molecular was 10 ns,it was calculated out according to this that the double-molecular quenching constants of DNA-EB and lead ions were 1.01×1013 and 4.0×1014 L/(mol·s)resp.,both of them were bigger than the maximum dynamic quenching constant,which was 2.0 ×1010 L/(mol·s),indicating that the fluorescent quenching induced by lead ions of DNA-EB belong to statistic quenching.According slope and extrapolation intercept,it was calculated out that the dissociation constant of DNA-EB and quencher was 1.13×10-5 mol/L.The binding site of lead ions on DNA-EB was 5.56 and their binding constant was 1.51×1029.[Conclusion] It was easy for lead ions to combine with DNA and induce the change of DNA conformation.

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