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Position: Home > Articles > Expression and purification of myostatin gene in chicken Heilongjiang Animal Science and Veterinary Medicine 2008 (7) 18-20

鸡肌抑素基因的原核表达及蛋白纯化

作  者:
蓝赐华;刘为民;梁梓森
单  位:
华南农业大学兽医学院;佛山科学技术学院动物医学系
关键词:
南海黄鸡;肌抑素基因;原核表达;蛋白纯化
摘  要:
利用实验室保存的重组质粒pMD18-MSTN重新构建原核表达质粒pET-32a-MSTN,将其转化到宿主菌大肠杆菌BL21上并进行诱导表达,再将所表达的融合蛋白进行纯化。结果表明:该基因在宿主菌中成功表达;目的蛋白被成功纯化,且纯度较高。
译  名:
Expression and purification of myostatin gene in chicken
作  者:
LAN Ci-hua1,2,3,LIU Wei-min1,LIANG Zi-sen2(1.Foshan University,Nanhai 528231,China;2.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;3.Guangdong Wen's Food Group Limited Corporation,Guang zhou 527400,China)
关键词:
Nanhai yellow chicken;myostatin;prokaryotic expression;protein purification
摘  要:
The recombinant plasmid pET-32a-MSTN was constructed according to the plasmid pMD18-MSTN preserved in our laboratory and then was transformed into BL21 to express target protein.The result showed that the gene can be expressed in E.coli.The expressed product was purified.Therefor the target protein can be purified and the purity was on a high level.

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