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Position: Home > Articles > Cloning and sequence analysis of myostatin gene of Nanhai Yellow Chicken Guangdong Agricultural Sciences 2007 (1) 65-68

南海黄鸡肌抑素基因的克隆及序列分析

作  者:
蓝赐华;刘为民;梁梓森
单  位:
佛山科学技术学院;华南农业大学兽医学院
关键词:
南海黄鸡;肌抑素;基因克隆;测序
摘  要:
从南海黄鸡的鸡胚中抽提总RNA,采用RT-PCR技术成功扩增到肌抑素基因的cDNA序列(第76~1 128位碱基),将目的片断克隆到PMD18-T-Simple载体,转化大肠杆菌DH5α,筛选阳性克隆质粒并进行测序分析。结果表明,克隆的目的片断由1 053个核苷酸组成,与预期结果一致,经与GenBank中已注册的序列AF019621对比,同源性为99.6%,其相应氨基酸的同源性为99.4%。
译  名:
Cloning and sequence analysis of myostatin gene of Nanhai Yellow Chicken
作  者:
LAN Ci-hua~(1,2),LIU Wei-min~1,LIANG Zi-sen~2(1.Foshan University,Foshan 528231,China;2.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China)
关键词:
Nanhai Yellow Chicken;myostatin;gene cloning;sequence analysis
摘  要:
Total RNA was extracted from the 14-day embryo of Nanhai Yellow Chicken.The fragment including No.76~1128 base of myostatin gene cDNA sequence was amplified by reverse transcription-polymerase chain reaction(RT-PCR),then was cloned into PMD18-T-Simple vector and was sequenced.The results showed that myostatin gene cDNA sequence cloned in the experiment consisted of 1 053 nucleotides and shared 99.6% homology with cDNA sequence of AF019621 in GenBank,the homology of myostatin amino acids squence between them was 99.4%.

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