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Position: Home > Articles > RAPD Analysis of Genetic Difference among the Parents and Hybrids Modern Agricultural Sciences 2008 (12) 101-104+117

罗非鱼亲本与杂交子代遗传差异的RAPD分析

作  者：
邓普恩;刘丽;刘楚吾
单  位：
广东海洋大学水产学院
关键词：
RAPD;奥利亚罗非鱼;尼罗罗非鱼;杂交子代;遗传差异
摘  要：
运用RAPD技术对奥利亚罗非鱼(Oreochromis aureus)、尼罗罗非鱼(Oreochromis niloticus)及其杂交子代(O.aureus ♂×O.niloticus ♀)的遗传多样性进行了研究。从40个RAPD随机引物中筛选出扩增效果好的引物13个,分别用于这3个罗非鱼群体的基因组DNA的扩增。结果显示,3个罗非鱼群体的平均多态性位点比率(P)分别为52.78%、44.36%、44.99%;个体间平均遗传相似系数(S)分别为0.9102、0.9121、0.9043;平均遗传距离(D)分别为0.0898、0.0879、0.0957;平均Nei基因多样性指数(H)分别为0.2248、0.2450、0.2293;平均Shannon信息指数(Hi)分别为0.0922、0.1040、0.1329。同时得到可用于鉴别3个群体的9条特异性分子标记。
译  名：
RAPD Analysis of Genetic Difference among the Parents and Hybrids
作  者：
DENG Pu- en LIU Li LIU Chu- wu (Fisheries College, Guangdong Ocean University, Zhanjiang, Guangdong 524025, China)
关键词：
RAPD; O.aureus; O.niloticus; hybrids; genetic difference
摘  要：
Genetic diversity and molecular evolution of 3 groups of Tilapia, O.aureus, O.niloticus, Hybrids, were detected using random amplified polymorphic DNA (RAPD) technique. A total 40 random primers were evaluated for the usefulness. Thereinto, 13 random primers were selected according to the effect of PCR of genomic DNA of 3 groups of Tilapia. The re- sults showed as the followings: the polymorphic loci ratio in O.aureus, O.niloticus, hybrids (P) were 52.78%,44.36% and 4.99% respectively; The mean genetic similarity index of intra- groups (S) were 0.9102, 0.9121 and 0.9043 respectively; the mean genetic distances of intra- groups (D) were 0.0898,0.0879 and 0.0957 respectively; Nei′ s gene diversity (H) were 0.2248, 0.2450 and 0.2293 respectively; and Shannon's information index (Hi) were 0.0922, 0.1040 and 0.1329 respectively. Meanwhile, 9 groups- specific markers in 3 groups were received.

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