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Position: Home > Articles > Callus culture and plant regeneration of Stylosanthes guianensis Acta Prataculturae Sinica 2007,16 (3) 136-138

柱花草愈伤组织培养与植株再生(简报)

作  者:
刘进平;吴发红;王丹;刘杨晶;朱涛
单  位:
华南热带农业大学农学院
关键词:
热研2号柱花草;组织培养;愈伤组织;植株再生
摘  要:
对热研2号柱花草无菌实生苗的胚轴与叶片切段进行愈伤组织培养研究。结果表明,MS+NAA 2 mg/L+BA 0.5 mg/L及MS+2,4-D 3 mg/L+BA 0.3~0.5 mg/L+CH 200 mg/L对叶片切段诱导效果最好。而下胚轴愈伤组织的诱导以MS+NAA 3 mg/L+BA 0.1~0.3 mg/L和MS+2,4-D 3 mg/L+BA 0.5 mg/L最优。愈伤组织在MS+BA 2.5 mg/L+NAA 0.1 mg/L进行分化培养,可实现高频植株再生。最适生根培养基为1/2MS+1.0mg/L IBA+0.5 mg/L IAA,生根率可达95.84%。
译  名:
Callus culture and plant regeneration of Stylosanthes guianensis
作  者:
LIU Jin-ping,WU Fa-hong,WANG Dan,LIU Yang-jing,ZHU Tao(Agronomy College,South China University of Tropical Agriculture,Danzhou 571737,China)
关键词:
Stylosanthes guianensis cv.Reyan2;tissue culture;callus;plant regeneration
摘  要:
Studies on callus culture and plant regeneration of Stylosanthes guianensis cv.Reyan 2 showed that the optimum callus induction media were MS+NAA 2 mg/L+BA 0.5 mg/L and MS+2,4-D 3 mg/L+BA 0.3-0.5 mg/L+CH 200 mg/L for leaf pieces,and MS+NAA 3 mg/L+BA 0.1-0.3 mg/L and MS+2,4-D 3 mg/L+BA 0.5 mg/L for hypocotyl segments from aseptic seedlings.High-frequency bud differentiation and plant regeneration was achieved on MS+BA 2.5 mg/L+NAA 0.1 mg/L.The best rooting medium was 1/2 MS +1.0 mg/L IBA+0.5 mg/L IAA.

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