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Position: Home > Articles > Enzymatic Extraction of Polysaccharides from Fermentation Mycelia of Gloeostereum incarnatum FOOD SCIENCE 2009,30 (13) 201-204

酶法提取榆耳菌丝体胞内多糖的工艺研究

作  者:
刘振春;蒋中华;李慧;段绪
单  位:
吉林农业大学食品科学与工程学院
关键词:
榆耳;多糖;酶解;纤维素酶
摘  要:
本实验主要对榆耳菌菌丝体多糖提取工艺进行研究,以提高产品的得率。通过单因素试验及正交试验,得纤维素酶提取榆耳菌丝体胞内多糖的最佳工艺条件:料水比1:60、pH5.3、温度43℃、酶解时间2.0h、加酶量2.5%,在此条件下,榆耳菌丝体胞子内多糖的提取率为4.86%。
译  名:
Enzymatic Extraction of Polysaccharides from Fermentation Mycelia of Gloeostereum incarnatum
作  者:
LIU Zhen-chun,JIANG Zhong-hua,LI Hui,DUAN Xu (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
关键词:
Gloeostereum insarnatum;polysaccharides;enzymatic hydrolysis;cellulase
摘  要:
Cellulase-catalyzed hydrolysis was used to extract polysaccharides from the collected mycelia of Gloeostereum incarnatum 105 after submerged fermentation. The extraction technology was optimized by orthogonal factorial design based on single factor test. The results showed that the optimal conditions for extracting polysaccharides from mycelia of Gloeostereum incarnatum 105 were as follows: solid/liquid ratio 1:60, pH 5.3, cellulose amount 2.5%, and hydrolysis temperature 43 ℃ for a hydrolysis duration of 2.0 h; under these conditions, the yield of polysaccharides reached 4.86%.

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