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当前位置: 首页 > 文章 > 彗星试验评价硝酸镧对蚕豆根尖细胞的遗传毒性 农业环境科学学报 2008,27 (06) 273-276
Position: Home > Articles > Evaluation of Genotoxicity Induced by Lanthanum Nitrate in the Root Tip Cells of Vicia faba by Comet Assay Journal of Agro-Environment Science 2008,27 (06) 273-276

彗星试验评价硝酸镧对蚕豆根尖细胞的遗传毒性

作  者：
杨定明;费红梅;景秀丽;谢艳;李宗芸
单  位：
徐州师范大学细胞与分子生物学研究所生命科学学院
关键词：
彗星实验;遗传毒性;硝酸镧;蚕豆根尖细胞
摘  要：
设置了0、1、4、16、64、256mg·L-16个浓度的硝酸镧梯度溶液,运用彗星电泳测试技术,以彗星尾长、彗星率、尾距和Olive尾距来评价La(NO3)3对蚕豆根尖细胞DNA的损伤。结果表明:与对照组相比,染毒组的彗星尾长、彗星率、尾矩和Olive尾矩随浓度增加而逐渐上升。一定剂量的稀土元素镧(≥1mg·L-1)可以使蚕豆根尖细胞DNA出现损伤,在1～64mg·L-1范围内随镧浓度的增加伤害程度加大,256mg·L-1时,遗传物质损伤严重,彗星尾长、尾距和Olive尾距反而下降,说明硝酸镧具有一定的细胞毒性和遗传毒性。
译  名：
Evaluation of Genotoxicity Induced by Lanthanum Nitrate in the Root Tip Cells of Vicia faba by Comet Assay
作  者：
YANG Ding-ming1, FEI Hong-mei1, JING Xiu-li1, XIE Yan1, LI Zong-yun1, 2 (1. Institute of Cellular and Molecular Biology, School of Life Science, Xuzhou Normal University, Xuzhou 221116, China; 2.Jiangsu Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Huai'an 223300, China)
关键词：
comet assay;genotoxicity;lanthanum nitrate;root tip cells of Vicia faba
摘  要：
Lanthanum is one of the rare chemicals which are widely used in agriculture in China because of their abilities to increase the yield and to improve quality of crops. But it also has aroused the people's attention to the environmental pollution by the rare-earth elements. To study the genotoxicity of lanthanum nitrate in the root tip cell of Vicia faba, six treated groups with different lanthanum nitrate content(0, 1, 4, 16, 64, 256 mg·L-1 respectively)were designed. Comet assay was used to detect DNA damage of root tip cells of Vicia faba according to the tail length, comet rate, tail moment, olive tail moment. The results showed that the tail length, comet rate, tail moment, olive tail moment of five treated groups were increased significantly with increasing of lanthanum nitrate concentration compared with the control group. It mean that DNA of root tip cell of Vicia faba can be damaged by lanthanum nitrate. In 1～64 mg·L-1 the damage extent was enhanced with increasing of lanthanum nitrate concentration. Genetic material was serious damaged in the concentration of 256 mg·L-1. On the contrary, the tail length, comet rate and tail moment were descent. It suggests that lanthanum nitrate has genotoxicity and cytotoxicity to root tip cells of Vicia faba.

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