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Position: Home > Articles > Histological Studies of Somatic Embryogenesis in Eruca sativa Mill Acta Horticulturae Sinica 2007,34 (1) 131-134

芸芥体细胞胚胎发生的组织细胞学研究

作  者:
张涛
单  位:
重庆师范大学生命科学学院
关键词:
芸芥;体细胞胚胎;组织细胞学
摘  要:
以芸芥子叶为外植体,在MS+2,4-D1.0mg.L-1培养基上培养2周后可诱导体细胞胚胎的发生。胚性愈伤组织在MS+2,4-D0.2mg.L-1培养基上大量增殖,体细胞胚胎在N6培养基上成熟后,可在附加0.1mg.L-1IBA的1/2MS培养基上生长。切片观察表明:芸芥体细胞胚胎为单细胞发生方式,与合子胚形成过程相似。芸芥胚性细胞具有细胞小、胞质浓、核大、核仁明显,排列紧密的特点,首先由单个细胞分裂形成2-细胞原胚,2-细胞原胚分裂成3-细胞原胚或4-细胞原胚,3-细胞原胚或4-细胞原胚继续分裂形成多细胞原胚,进一步发育成一个成熟的体细胞胚胎。
译  名:
Histological Studies of Somatic Embryogenesis in Eruca sativa Mill
作  者:
ZHANG Tao1,2(1College of Life Sciences,Chongqing Normal University,Chongqing 400047,China;2Gansu Academy ofAgricultural Sciences,Lanzhou 730070,China)
关键词:
Eruca sativa;Somatic embryos;Histological
摘  要:
Somatic embryos were induced from cotyledon explants cultured on Murashige and Skoog(MS)medium supplemented with 1.0 mg·L-1 2,4-D after two weeks of culture.Embryogenic callus proliferated profusely on MS medium containing 0.2 mg·L-1 2,4-D.Somatic embryos matured on N6 medium and developed into plantlets by culturing the mature somatic embryos on 1/2MS medium containing 0.1 mg·L-1indole-3-butyric acid.Section and microscopic observation of different developmental stages revealed that somatic embryogenesis was similar to that of the zygotic embryo.It originated from a single embryogenic cell,which had bigger nuclei,thick cytoplasm,clear nucleoli and denser order.The embryogenic cell first divided into two-celled stage proembryo,then three-or four-celled stage proembryo.Three-or four-celled stage proembryo developed into multicellular proembryo,then developed into mature somatic embryo.

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