中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 芸芥自交亲和相关基因的差异显示及表达分析 中国油料作物学报 2014,36 (5) 580-585
Position: Home > Articles > Differential display and expression analysis of self- compatibility associated gene in Eruca sativa Chinese Journal of Oil Crop Sciences 2014,36 (5) 580-585

芸芥自交亲和相关基因的差异显示及表达分析

作  者：
方彦;孙万仓;武军艳;曾秀存;刘自刚;杨刚;杨宁宁
单  位：
甘肃农业大学农学院;甘肃省油菜工程技术研究中心
关键词：
芸芥;自交亲和基因;DDRT-PCR;表达分析
摘  要：
为分离和筛选芸芥自交亲和相关基因,以芸芥自交亲和系(SC)和自交不亲和系(SI)开花前及开花后的花药和柱头为试材,利用mRNA差异显示技术筛选芸芥自交亲和相关差异表达基因,结合实时荧光定量PCR技术验证差异表达基因在SI和SC开花前后不同时期的表达水平差异。结果共筛选得到了11条差异片段。差异表达基因包含木葡聚糖半乳糖基转移酶、DnaJ伴侣蛋白、果胶酸裂解酶家族蛋白、40S核糖体蛋白s19-1和假设蛋白等。木葡聚糖半乳糖基转移酶基因在SC开花前柱头中表达量最大,其表达量是SI开花前柱头中的4.41倍;SC8假设蛋白基因在芸芥SC开花后花药中表达量最大,是SI开花后花药中的23.98倍。初步推测这些差异基因可能在芸芥SI和SC性状调控过程中发挥重要作用。
译  名：
Differential display and expression analysis of self- compatibility associated gene in Eruca sativa
作  者：
FANG Yan;SUN Wan-cang;WU Jun-yan;ZENG Xiu-cun;LIU Zi-gang;YANG Gang;YANG Ning-ning;Agricultural College,Gansu Agricultural University;Instrumental Research and Analysis Center of Gansu Agricultural University;Rape Engineering and Technology Research Center of Ganxu Provincial;
关键词：
Eruca sativa;;Self-compatibility gene;;DDRT-PCR(differential display reverse transcriptase polymerase chain reaction);;Expression analysis
摘  要：
To screen the genes associated with the self- compatibility of Eruca sativa,RNAs isolated from anther and stigma of pre- bloom and after flowering were used to investigate differential gene expression in SC and SI of E. sativa through DDRT- PCR( differential display reverse transcriptase polymerase chain reaction) method.The key differential genes before and after flowering in SI and SC were confirmed with real- time PCR. Results showed that 11 differential genes were obtained coding xyloglucan galactosyltransferase,chaperone protein dnaJ,pectate lyase family protein,40 S ribosomal protein s19- 1,hypothetical protein,etc. Xyloglucan galactosyltransferase gene in SC stigma had the highest expression before flowering and were 4. 41 folds than that in SI stigma.Hypothetical SC8 gene in SC anther had the highest expression after flowering and were 23. 98 folds than in SI anther. Results indicated that the above genes might have played important roles in SI and SC characteristics of regulation of E. sativa.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46