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当前位置: 首页 > 文章 > 水稻低直链淀粉含量基因Wx~(mq)的KASP标记开发与利用 分子植物育种 2019 (24) 8125-8131
Position: Home > Articles > Development and Utilization of KASP Marker for Low Amylose Content Gene, Wx~(mq), in Rice(Oryza Sativa L.) Molecular Plant Breeding 2019 (24) 8125-8131

水稻低直链淀粉含量基因Wx~(mq)的KASP标记开发与利用

作  者：
牛付安;周继华;曹黎明;程灿;涂荣剑;胡雪娇;杨佳;孙滨;罗忠永;赵志鹏;王新其;储黄伟
单  位：
上海市农业科技服务中心;上海市农业科学院作物育种栽培研究所;上海海洋大学食品学院;江西农业大学农学院
关键词：
水稻(Oryza sativa);直链淀粉含量;Wxmq;KASP
摘  要：
拥有低直链淀粉含量基因Wx~(mq)的软米具有适口和膨化性好、软而不烂、富有弹性、回生程度小、冷后不易变硬等优点。为了提高优质软米的选育效率,根据Wx~(mq)基因第4外显子的SNP1突变位点开发了一个高通量分子标记Wx~(mq)-KASP,利用该标记对粳稻软米品种‘南粳9108’、中等直链淀粉含量粳稻保持系‘申01B’及其杂交F_2代群体进行基因分型,在F_2群体83个单株中,有21个单株表现出与‘南粳9108’相同的基因型,20个单株与‘申01B’基因型相同,而42个单株表现为杂合基因型。利用前人开发的CAPS标记对‘南粳9108’、‘申01B’及其杂交F_2群体的Wx基因进行了基因型检测,所得结果与Wx~(mq)-KASP检测结果完全一致。该研究表明KASP标记Wx~(mq)-KASP可以高效应用于水稻低直链淀粉含量种质资源鉴定及分子标记辅助选择育种。
译  名：
Development and Utilization of KASP Marker for Low Amylose Content Gene, Wx~(mq), in Rice(Oryza Sativa L.)
作  者：
Niu Fuan;Zhou Jihua;Cao Liming;Cheng Can;Tu Rongjian;Hu Xuejiao;Yang Jia;Sun Bin;Luo Zhongyong;Zhao Zhipeng;Wang Xinqi;Chu Huangwei;Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences;School of Agricultural Sciences, Jiangxi Agricultural University;College of Food Sciences and Technology, Shanghai Ocean University;Shanghai Municipal Agricultural Science&Technology Service Center;
关键词：
Rice(Oryza sativa);;Amylose content;;Wxmq;;KASP
摘  要：
Soft rice with low amylose content gene Wx~(mq)has the advantages of palatability, good puffing, soft but not rotten, elasticity, low degree of retrogradation and hardening after cold. In order to improve the breeding efficiency of soft rice with high quality, a high throughput molecular marker, Wx~(mq)-KASP, developed based on the SNP1 site in exon 4 of Wx~(mq)gene, was used to genotype the japonica rice variety 'Nanjing 9108' with low amylose content, japonica rice maintainer Line 'Shen01B' with medium amylose content and their F_2 population. Of eighty-three individual plants in F_2 population, 21 and 20 individuals, respectively, have the similar genotype as' Nanjing 9108' and 'Shen01B', whereas 42 individuals were heterozygous. The genotype of 'nanjing 9108', 'shen01B' and their hybrid F_2 population was also detected using the CAPS marker developed by predecessors, and the results obtained were completely consistent with the results of Wx~(mq)-KASP. This study indicated that KASP marker Wx~(mq)-KASP could be effectively applied in molecular marker-assisted selection breeding of low amylose content rice.

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