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Position: Home > Articles > Construction of Prokaryotic Expression Plasmid with G and N Genes of Rabies Virus and Co-Expression of the Two Genes Journal of Jilin Agricultural University 2006,28 (3) 313-316

狂犬病病毒糖蛋白、核蛋白融合基因原核质粒的构建与表达

作  者:
姜海龙;钱爱东
单  位:
吉林农业大学动物科技学院
关键词:
狂犬病病毒;糖蛋白;核蛋白;原核表达质粒;共表达
摘  要:
试验将编码狂犬病病毒糖蛋白、核蛋白融合基因克隆到原核表达载体pET-32a(+)中,构建狂犬病病毒原核表达质粒。将该质粒转化到大肠杆菌BL21中,经IPTG诱导表达出约2.09×104D的狂犬病病毒糖/核融合基因编码的蛋白质。Western-blotting检测结果表明,该融合蛋白质具有免疫原性。
译  名:
Construction of Prokaryotic Expression Plasmid with G and N Genes of Rabies Virus and Co-Expression of the Two Genes
作  者:
JIANG Hai-long,QIAN Ai-dong(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
关键词:
rabies virus;glycoprotein;neucleoprotein;prokaryotic expression plasmid;co-expression
摘  要:
The experiment was conducted to construct prokaryotic expression plasmid containing the G and N melt genes of rabies virus through cloning the genes of rabies virus into pET-32a(+).After transforming the plasmid into E. coli BL21,the bacteria were induced by IPTG and the protein was detected with western-blotting test.The result indicated that the two genes could be co-expressed by the prokaryotic expression plasmid,which will supply foundation for the preparation of the antigen use to diagnose rabies.

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