中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 狂犬病病毒中和抗体ELISA技术的建立 中国动物检疫 2011,28 (1) 48-51
Position: Home > Articles > Indirect ELISA for Determination of Neutralizing Antibody of Rabies Virus China Animal Health Inspection 2011,28 (1) 48-51

狂犬病病毒中和抗体ELISA技术的建立

作  者：
罗金燕;王水明;李向东;沈阳;邱亚峰;史子学;丁铲;艾峰;刘学辉;张帆;刘佩红;马志永
单  位：
南京出入境检验检疫局;中国农业科学院上海兽医研究所;上海动物疫病预防控制中心
关键词：
狂犬病病毒;G蛋白;中和抗体;间接ELISA
摘  要：
将狂犬病病毒(RV)糖蛋白(G蛋白)中和抗原表位串联表达的重组蛋白作为抗原,建立了检测RV中和抗体的间接ELISA技术。结果表明,最佳抗原包被量为2μg/孔,被检血清最佳稀释倍数为1:200。该方法与快速荧光灶抑制试验(RFFIT)的阳性符合率为88%,阴性符合率为96%。特异性试验表明,该抗原不与犬腺病毒I型、犬细小病毒、犬瘟热病毒、犬副流感病毒和犬冠状病毒阳性血清发生交叉反应,具有良好的特异性。板内和板间重复性试验的平均变异系数分别为2.7%和4.2%,具有良好的重复性,为动物RV中和抗体检测提供了简单快捷的检测方法。
译  名：
Indirect ELISA for Determination of Neutralizing Antibody of Rabies Virus
作  者：
Luo Jinyan1,Wang Shuiming2,Li Xiangdong1,Shen Yang1,Qiu Yafeng1,Shi Zixue1,Ding Chan1,Ai Feng2,Liu Xuehui2,Zhang Fan2,Liu Peihong3,Ma Zhiyong1(1.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241;2.Nanjing Entry-Exit Inspection and Quarantine Bureau,Nanjing,210001;3:Shanghai Animal Disease Control Center,Shanghai,201103)
关键词：
Rabies virus;G protein;Neutralizing antibody;Indirect ELISA
摘  要：
An indirect ELISA assay for determination of neutralizing antibody of rabies virus was established by using the recombinant G protein as antigen.The recombinant G protein was obtained by expressing the spliced gene fragments that encode polypeptides comprising the linear neutralization sites of the G protein.The results showed that the amount of recombinant G protein for coating was 2 μg per well and the dilution for the detecting sample was 1:200.The positive coincidence rate and negative coincidence rate of samples between the established ELISA and rapid fluorescent focus inhibition test(RFFIT) was 88% and 96% respectively.The ELISA did not cross-react with sera positive for canine adenovirus type I,canine parvovirus,canine distemper virus,canine parainfluenza virus and canine coronavirus,showing good specificity.The intra-assay and inter-assay coefficient of standard variation was 2.7% and 4.2% respectively.The indirect ELISA would be useful for detecting neutralizing antibody of rabies virus in animals.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46