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当前位置: 首页 > 文章 > 白腐真菌F-9的产酶研究 北方园艺 2009 (7) 79-82
Position: Home > Articles > Characterization of Ligninolytic Enzymes Production of a White-rot Fungus F-9 Northern Horticulture 2009 (7) 79-82

白腐真菌F-9的产酶研究

作  者：
戴珅;林书欣;刘洪涛;索凡;朱启忠;张小葵
单  位：
山东大学威海分校
关键词：
白腐真菌;生长;产酶;木质素
摘  要：
白腐真菌F-9是新分离得到的一株白腐真菌,分别采用PDA固体培养基、液体天然培养基和液体人工培养基对它进行培养,并对它的产酶情况进行研究。研究结果表明:F-9在PDA培养基中的生长速率较快,可达到8 mm/d;在液体天然培养基中的生长速率和产酶情况也比较理想,LiP酶活力可达到341 U/L,MnP可达到264 U/L;在液体人工培养基中LiP和MnP活性达到最大的时间比在天然培养基中晚2~3 d,但酶的活力都有很大提高,LiP酶活力可达到619 U/L,MnP酶活力可达到443 U/L。
译  名：
Characterization of Ligninolytic Enzymes Production of a White-rot Fungus F-9
作  者：
DAI Shen,LIN Shu-xin,LIU Hong-tao,SUO Fan,ZHU Qi-zhong,ZHANG Xiao-kui(Shandong University at Weihai,Weihai,Shandong 264209,China)
关键词：
White-rot fungus F-9;Growth;Enzyme;Lignin
摘  要：
White-rot fungus F-9 was the fungus isolated newly.Used the PDA culture medium,the natural culture medium on liquid and the artificial culture medium on liquid carries on the raise on F-9 separately,and studied the production of enzyme.The study illustrated that its growth rate in PDA culture medium was quick,may reach 8 mm/d;and its growth rate and production of enzyme in natural culture medium on liquid quit to be also ideal,LiP was 341 U/L,MnP was 264 U/L;the peak activities of LiP and MnP produced by F-9 in the artificial culture medium on liquid appear later than the fungus in natural culture medium on liquid about 2~3 d,but the activities of the two enzyme were both greatly improved,LiP was 619 U/L,MnP was 443 U/L.Judging from the result,if optimize the train condition of F-9 further,it ought to get much better lignin degradation effect.

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