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Position: Home > Articles > Effect of Temperature, pH and Salts on Plantaricin L-1 to Listera monocytogenes FOOD SCIENCE 2006,27 (2) 121-125

温度、pH及盐对植物乳杆菌素L-1作用单核细胞增生李斯特氏菌的影响

作  者:
周伟;李平兰;周康;吕燕妮
单  位:
中国农业大学食品科学与营养工程学院
关键词:
植物乳杆菌素;单核细胞增生李斯特氏菌;温度;pH;盐
摘  要:
本试验分析了透析除盐后的植物乳杆菌素L-1的效价和作用方式,测定了在食品加工过程中常涉及到的温度、pH和盐等生化条件对其作用单核细胞增生李斯特氏菌的影响,并初步探索了pH和盐对植物乳杆菌素L-1吸附作用的影响。结果表明:透析除盐后的植物乳杆菌素L-1的效价可达1280AU/ml,作用方式为杀菌;低温下144h内可以控制住初始菌数,高温下可以短时间内迅速降低初始活菌数;在pH7.0下,该细菌素的抑菌效果最好;无论是TSBYE培养基中还是磷酸缓冲液中,试验所采用的四种盐对植物乳杆菌素L-1的杀菌作用都有一定的拮抗作用,各盐之间和同种盐内不同浓度间差异不显著;对吸附作用的初步研究发现pH对植物乳杆菌素的吸附作用有一定影响,而盐对其影响不显著。
译  名:
Effect of Temperature, pH and Salts on Plantaricin L-1 to Listera monocytogenes
作  者:
ZHOU Wei,LI Ping-lan*,ZHOU Kang,LU Yan-ni (College of Food Science and Nutritional Engineering, China Agricultrual University, Beijing 100083, China) .
关键词:
plantaricin;L.monocytogenes;temperature;pH;salts
摘  要:
Plantaricin L-1, an anti-listeria bacteriocin, obtained by precipitation with 80% ammonium sulphate is 1280AU/ml. The mode of action is bactericidal. Influence of temperature, pH and salts on inhibitory activity of plantaricin L-1 showed: that the inhibitory activity can last 144h at lower temperature, and the CFU/ml can be decreased at higher temperatures. pH7.0 was optimal, a significant reduction in activity was observed at acidic pH values; Salts in TSBYE broth and phosphate buffer (5mmol/L, pH7.0) tested can reduce the activity of plantaricin L-1, the difference between various salts was not significant, as well as the difference between three density of the same salt. Adsorption of plantaricin L-1 was influenced by the pH in some degree, however, salts didn’t change the adsorption of plantaricin L-1.

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