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当前位置: 首页 > 文章 > 酶解法制备草鱼鱼鳞多肽及其清除羟自由基的研究 食品科学 2010,31 (7) 168-172
Position: Home > Articles > Enzymatic Preparation and Hydroxyl Free Radical Scavenging Activity of Polypeptides from Grass Carp Scales FOOD SCIENCE 2010,31 (7) 168-172

酶解法制备草鱼鱼鳞多肽及其清除羟自由基的研究

作  者：
杜云建;赵玉巧;李念念
单  位：
淮海工学院海洋学院
关键词：
草鱼鱼鳞;多肽;清除自由基作用;响应面
摘  要：
采用复合蛋白酶酶解草鱼鱼鳞制备多肽并研究其清除羟自由基能力,考察酶解条件对多肽清除羟自由基的影响,通过单因素试验和响应面试验优化得到清除羟自由基的多肽酶解工艺条件为:底物质量浓度为6g/100mL,酶解时间2.11h,加酶量为5.22g/100mL,酶解温度49.33℃,最高清除率为99.24%。层析分离的结果有5个洗脱峰,第4个峰的羟自由基清除率最高,达到98.34%。
译  名：
Enzymatic Preparation and Hydroxyl Free Radical Scavenging Activity of Polypeptides from Grass Carp Scales
作  者：
DU Yun-jian,ZHAO Yu-qiao,LI Nian-nian(College of Ocean,Huaihai Institute of Technology,Lianyungang 222005,China)
关键词：
carp scale;polypeptide;hydroxyl free radical scavenging activity;response surface analysis
摘  要：
Complex proteases were used to hydrolyze grass carp scales for preparing polypeptides with high hydroxyl free radical scavenging activity in the present study.To maximize hydroxyl free radical scavenging activity of polypeptides prepared from grass carp scales,three crucial hydrolysis parameters were investigated by response surface analysis based on central composite design.Besides,hydrolysis products of grass carp scales obtained were purified/fractionated by Sephadex G-15 column chromatography and the resulting fractions were evaluated for hydroxyl free radical activity.The optimal values of the above parameters for improved hydrolysis of grass carp scales were determined as follows:substrate concentration 6g/100 mL,enzyme dose 5.22 g/100 mL and reaction temperature 49.33 ℃ for a hydrolysis duration of 2.11 h.Under the optimized parameters,a maximum hydroxyl free radical scavenging activity of hydrolyzed grass carp scales of 99.24% was obtained.Sephadex G-15 column chromatographic separation resulted in 5 fractions and the highest scavenging rate(98.34%) against hydroxyl free radicals was observed in the fourth fraction.

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