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草鱼鱼鳞中活性胶原蛋白提取工艺及参数优化

作  者:
王艳;汪海波;桂萌;王孟津;张含俊;刘良忠
单  位:
武汉工业学院食品科学与工程学院
关键词:
草鱼鱼鳞;胶原蛋白;提取;参数优化
摘  要:
以草鱼鱼鳞为原料,在低于蛋白变性温度的条件下提取酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC),并对鱼鳞原料的前处理方法、胶原蛋白提取工艺进行优化。结果表明,在鱼鳞原料的前处理工艺中,以0.1mol/L的Na2CO3溶液为试剂脱除原料中杂蛋白的最佳工艺条件为室温、料液比1:40(g/mL),300r/min搅拌处理3次,每次6h;以0.3mol/L的EDTA为试剂脱除原料中矿物质杂质的最佳工艺条件为室温、料液比1:60,搅拌处理3次,每次12h;ASC的最佳提取工艺条件为:提取温度不高于25℃,料液比1:60,醋酸浓度0.8mol/L,提取3次,每次24h;PSC的最佳提取工艺条件为:胃蛋白酶用量为鱼鳞原料质量的3.0%、醋酸浓度0.8mol/L、料液比1:30、提取温度4℃、提取2次,每次24h。
译  名:
Extraction Optimization of Collagen from Grass Carp Scales
作  者:
WANG Yan,WANG Hai-bo*,GUI Meng,WANG Meng-jin,ZHANG Han-jun,LIU Liang-zhong(School of Food Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China)
关键词:
the scales of grass carp;collagen;extraction;optimal parameters
摘  要:
Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were extracted from the scales of grass carp at low temperature,and optimal extraction parameters were explored.The optimal parameters for removing impurity proteins from fish scales with 0.1 mol/L Na2CO3 were material/liquid ratio of 1:40 for mixing 3 times for 6 h each time at room temperature.The optimal parameters for removing mineral impurities with 0.3 mol/L EDTA were material/liquid ratio of 1:60 for mixing 3 times 12 h for each time at room temperature.The optimal parameters for ASC extraction were material/liquid ratio of 1:60 for extraction with 0.8 mol/L acetic acid 3 times for 24 h each time at 25 ℃.The optimal parameters for PSC extraction were amount of pepsin on the basis of the weight of fish scales,3.0%;acetic acid concentration,0.8 mol/L;and material/liquid ratio 1:30 for twice extraction at 4℃ for 24 h each time.

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