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当前位置: 首页 > 文章 > 猪萨佩罗病毒间接免疫荧光方法的建立与初步应用 湖南农业大学学报(自然科学版) 2017 (4) 423-426
Position: Home > Articles > Establishing the indirect immunofluorescence assay for antibody detection from Porcine sapelovirus Journal of Hunan Agricultural University(Natural Sciences) 2017 (4) 423-426

猪萨佩罗病毒间接免疫荧光方法的建立与初步应用

作  者：
彭旺;唐小明;葛猛;杨涛涛;屈泰龙;余兴龙
单  位：
湖南农业大学动物医学院;湖南省动物疫病预防控制中心
关键词：
猪萨佩罗病毒;间接免疫荧光;抗体检测;湖南
摘  要：
以分离的猪萨佩罗病毒PSV Hu N1/2016株感染PK15细胞,制备抗原板,建立检测猪血清中PSV抗体的间接免疫荧光(IFA)方法。结果表明:一抗最佳稀释浓度为1∶300;FITC标记的羊抗猪荧光二抗最佳稀释浓度为1∶300。该方法特异性强,敏感度高,既能将PSV抗体与PRRSV、FMDV、PCV、CSFV抗体区分开,也可以检测到中和效价0.128的阳性血清。用该方法检测湖南省部分规模化猪场208份临床血清样品的总阳性率为68%,表明PSV在湖南省流行普遍,且其感染主要发生在保育阶段。
译  名：
Establishing the indirect immunofluorescence assay for antibody detection from Porcine sapelovirus
作  者：
PENG Wang;TANG Xiao ming;GE Meng;YANG Taotao;QU Tailong;YU Xinglong;College of Veterinary Medicine,Hunan Agriculture University;Animal Disease Control Center of Hunan Province;
关键词：
Porcine sapelovirus;;indirect immunofluorescence assay;;antibody detection;;Hunan province
摘  要：
Indirect immunofluorescence assay(IFA) for detecting antibody of Porcine sapelovirus(PSV) from porcine serum was established by employed the strain PSV Hu N1 cultured in PK–15 cells as antigen coating in plate. The primary antibody and fluorescent secondary antibody in the test were diluted to 300 times. The results showed that the approach not only had high specificity and sensitivity which could discriminate PSV from other porcine virus antigens(such as PRRSV, FMDV, PCV, and CSFV), but also could detect positive serum sample with concentration low to 0.128 SN titer. The fact detected by the established method that 68% samples out of 208 serums from commercial farms scattered in Hunan province were positive indicated that the PSV was widely prevalent in the region, and the infection was mainly occurred in the weaning period.

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