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马病毒性动脉炎病毒中和试验的建立以及与ELISA方法的比较研究

作  者:
张海明;郑丽兰;段晓冬;相文华;沈丹;薛红;梁卓粤;彭南秀;程忠;彭聪
单  位:
广州市动物卫生监督所;中国农业科学院哈尔滨兽医研究所
关键词:
马病毒性动脉炎;病毒中和试验;酶联免疫吸附试验;比较
摘  要:
参照世界动物卫生组织(OIE)《陆生动物诊断与疫苗手册》,建立了马病毒性动脉炎病毒中和试验,并采用马病毒性动脉炎病毒中和试验(Virus Neutralization Test,VNT)和酶联免疫吸附试验(ELISA)两种方法,对广东省部分地区马场共182匹马血清和7份标准阴阳性血清进行马病毒性动脉炎抗体的检测,并对两种方法进行比较分析,结果两种方法的阳性符合率和阴性符合率分别为43.48%和96.34%,总符合率为93.12%;以我们建立的VNT作为马病毒性动脉炎抗体检测的"金标准",试验中所用的ELISA方法的敏感性和特异性分别为71.43%和93.96%。因此,我们认为在马病毒性动脉炎抗体检测中,ELISA的敏感性以及与VNT的阳性符合率较低,不适合作为VNT的替代方法。
译  名:
Establishmant of EVA Virus Neutralization Test and Its Comparison with ELISA for Detection of EVA
作  者:
Zhang Haiming 1 ,Zheng Lilan 1 ,Duan Xiaodong 1 ,Xiang Wenhua 2 ,Shen Dan 1 , Xue Hong 1 ,Liang Zhuoyue 1 ,Peng Nanxiu 1 ,Cheng Zhong 1 ,Peng Cong 1 (1.GuangzhouAnimal Disease Prevention and Control Center,GuangzhouAnimal Health Supervision Institute,Guangzhou,Guangdong,510440,China;2. Harbin Veterinary Research Institute,CAAS, Harbin,Heilongjiang,150001,China)
关键词:
EVA;VN;ELISA;comparison
摘  要:
The equine viral arteritis virus neutralization tes(tVNT)was developed according to OIE《Manual of Diagnosis Tests and Vaccines for Terrestrial Animals》. Total 182 horse sera from Guangdong province and 7 standard negative and positive sera were tested with EVA VNT and ELISA,respectively. The result showed that the positive coincidence rate and negative coincidence rate of the two tests were 43.48% and 96.34% respectively,and the total coincidence rate was 93.12%;Sensitivity and specificity of the ELISA were 71.43% and 93.96% respectively,as the VNT was taken as "Gold Standard". In conclusion,the ELISA was not suitable for EVA antibody test.

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