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当前位置: 首页 > 文章 > 戴氏虫草无性型的研究——Ⅱ多糖 西南农业学报 1993 (1) 27-32
Position: Home > Articles > STUDIES ON THE ANAMORPH OF CORDYCEPS TAII II POLYSACCHARIDE Southwest China Journal of Agricultural Sciences 1993 (1) 27-32

戴氏虫草无性型的研究——Ⅱ多糖

作  者：
焦彦朝;梁宗琦;刘爱英
单  位：
贵州农学院虫生真菌研究室;贵州省兽药监察所
关键词：
虫草属;戴氏虫草无性型;多糖;免疫调节剂;培养基
摘  要：
本文报道了戴氏虫草(Cordyceps taii Liang et Liu)的无性型,戴氏绿僵菌(Metarhizium taii Liang et Liu)88-601菌株水溶性胞外多糖(CD-1)的免疫调节作用和多糖的组成及液体培养的条件。结果表明,CD-1经提取、分离、纯化表明它是均一性的葡聚糖,分子量约200,000,产量为13.5 g/l(纯多糖)。有免疫调节作用。菌丝生长的适宜温度范围为25～29℃。37℃时分生胞子不萌发,菌丝产量和粗多糖产量最高的培养基为黄豆粉葡萄糖培养液(SGM)(分别为1.24g/100ml,16.75g/l)。粗多糖纯度可达80％。
译  名：
STUDIES ON THE ANAMORPH OF CORDYCEPS TAII II POLYSACCHARIDE
作  者：
Jiao Yanchao Liang Zongqi Liu Aiying (Laboratory of Entomogenous Mycology, Guizhou Agri. College, Guiyang, China 550025)
关键词：
Cordyceps Anamorph of Cordyceps taii Polysaccharide Immunomodulator Culture media
摘  要：
The cordyceps polysaccharides have important effects on inhibitor of cancers, radio-protection and enhancement of immune response. These substances have little or no toxicity. But many of them are intracellular. Few of them are extracellar, but water-insoluble reported so far. These situation increases the difficulties of industral fermentation, because the polysaccharides mixed wi th mycelia. In this paper, a new species Metarhizium tail Liang et Liu is an anamoiph of Cordyceps taii Hang et Liu. The composition and the enhancement of inmune response of the water-soluble extracellular polysaccharides (CD-I) in this new species and liquid cultures at different conditions are reported. The main results are as follows; CD-1 purified and separated, showed a homogenous pattern in gel filtration. The molecular weight was estimated by gel filtration to be 200>000. It was composed of D-glucose. CD-1 is shown enhancement of immune response by lymphocyte transfromation. The variation of mycelial growth curve is obviously affected by static culture and shaking culture. The optimum temper ature ranges is 25~29℃. The conidia have no growth at 37℃. This result suggestes that the conidia is safety to man, livestock, poultry. The medium of the heightest yield of hyphae and CD-1 is soybean glucose medium (SGM) . The yield of hyphae can reach 1.24g/100ml and the yield of CD-1 can reach 13.5g/l (pure polysaccharide). It is high as comperad with other cord yceps polysaccharides.

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