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当前位置: 首页 > 文章 > 香蕉分化芽BBTVDAS-ELISA检测方法的建立和应用 植物保护 2014 (5) 117-120
Position: Home > Articles > Establishment and application of DAS-ELISA method for detection of Banana bunchy top virus in banana differentiated shoots Plant Protection 2014 (5) 117-120

香蕉分化芽BBTVDAS-ELISA检测方法的建立和应用

作  者：
周朋;余乃通;章绍延;王健华;胡加谊
关键词：
香蕉束顶病毒;DAS-ELISA;病毒检测
摘  要：
为了解海南海口香蕉分化芽携带香蕉束顶病毒(Banana bunchy top virus,BBTV)的情况,本研究建立了双抗夹心酶联免疫反应(DAS-ELISA)检测法,对来自海南地区组培厂的香蕉分化芽进行检测。检测结果表明,6个品种1 852个香蕉分化芽平均带毒率为2.1%,其中‘皇帝蕉’为1.98%,‘粤科1号’为2.07%,‘广粉蕉’为1.98%,‘大蕉’为6.25%,‘218’为1.89%,‘巴西蕉’为2.25%。为了进一步验证ELISA结果的可靠性,随机选取12个阳性样品提取总DNA进行PCR鉴定。鉴定结果显示,12个样品中11个检测出有BBTV,表明DAS-ELISA方法的准确率较高,与分子检测结果基本一致,可以胜任日常香蕉组培苗BBTV的检测。
译  名：
Establishment and application of DAS-ELISA method for detection of Banana bunchy top virus in banana differentiated shoots
作  者：
Zhou Peng;Yu Naitong;Zhang Shaoyan;Wang Jianhua;Hu Jiayi;Wang Xiang;Liang Jie;Liu Zhixin;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science;College of Agriculture,Hainan University;
关键词：
Banana bunchy top virus;;DAS-ELISA;;virus detection
摘  要：
In order to understand the situation of Banana bunchy top virus(BBTV)infection in banana differentiated shoots in Hainan,1 852 shoot samples from6 banana varieties were detected by DAS-ELISA method.The results showed that the average BBTV-carrying rate was 2.1%,while 1.98%of‘Huangdi'banana,2.07%of‘Yueke No.1'banana,1.98%of‘Guangfen'banana,6.25%of Musa sapientum,1.89% of‘218'banana,and 2.25% of‘Brazil'banana,respectively.To further confirm the accuracy of DAS-ELISA result,12 BBTV-carrying samples were selected for molecular detection by PCR.The results showed that 11 of them were BBTV positive,indicating DAS-ELISA method with the high accuracy can be used for BBTV detection in banana differentiated shoots.

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