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当前位置: 首页 > 文章 > 红地球葡萄GLRaV-3的茎尖培养脱毒及RT-PCR检测 中国农学通报 2011,27 (4) 198-202
Position: Home > Articles > Shoot Tip Tissue Virus-free Culture and RT-PCR Detection of GLRaV-3 in Red Globe Grape Chinese Agricultural Science Bulletin 2011,27 (4) 198-202

红地球葡萄GLRaV-3的茎尖培养脱毒及RT-PCR检测

作  者：
韩艳婷;石雪晖;杨国顺;王先荣;刘昆玉;徐丰;倪建军
单  位：
湖南神州庄园葡萄酒业有限公司;湖南农业大学园艺园林学院
关键词：
红地球葡萄;茎尖培养;脱毒;葡萄卷叶病;RT-PCR
摘  要：
笔者以带GLRaV-3病毒的红地球为试材,研究了6-BA、NAA、IBA、KT不同配组及茎尖大小对茎尖培养的影响,并利用RT-PCR技术对部分红地球组培苗进行了GLRaV-3病毒检测。研究结果表明:红地球葡萄茎尖培养脱毒最佳茎尖初始培养基为:1/2MS+6-BA0.5mg/L+KT0.5mg/L+NAA0.2mg/L,最佳茎尖分化培养基为:B5+KT1.0mg/L+NAA0.2mg/L;茎尖大小与成活率成正比,与脱毒率成反比;运用RT-PCR检测技术进行了脱毒后组培苗的检测,得到了GLRaV-3的特异片段为300bp,获得了28株脱毒苗,平均脱毒率为43%。
译  名：
Shoot Tip Tissue Virus-free Culture and RT-PCR Detection of GLRaV-3 in Red Globe Grape
作  者：
Han Yanting1,Shi Xuehui1,Yang Guoshun1,Wang Xianrong2,Liu Kunyu1,Xu Feng1,Ni Jianjun1(1College of Horticulture and Landscape,Hunan Agricultural Univerdity,Changsha 410128;2Hunan Shenzhou Zhuangyuan Grape Wine Industry Limited Company,Li County Hunan 415500)
关键词：
red globe grape;shoot tip culture;virus elimination;GLRaV-3;RT-PCR
摘  要：
Effects of the stem-apex size and different combinations among 6-BA,NAA,IBA and KT on shoot tip culture in vitro were studied by using red globe grape infected with GLRaV-3 virus.GLRaV-3 was detected early in some of red globe grape of plantlets in vitro with RT-PCR technique.The results showed that the best suitable initial medium was 1/2MS+6-BA 0.5 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L.The optimum medium for shoot differentiation was B5+KT 1.0 mg/L+NAA0.2 mg/L.Plants free of GLRaV-3 were found.The size of excised shoot tip was proportional to the survival sate and inverse ratio to the virus elimination rate.TheRT-PCR detection technology was used for the GLRaV-3 detection and a 300 bp specific fragment was effectively amplified.The virology detection showed that virus-free red globe grape were obtained in 28 and the average rate of virus elimination was 43%.

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