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当前位置: 首页 > 文章 > 黄芪甲苷缓解对乙酰氨基酚诱导的肝脏氧化应激损伤 安徽科技学院学报 2019 (5) 34-39
Position: Home > Articles > Astragaloside IV Ameliorates Acetaminophen-induced Liver Damage by Oxidative Stress Journal of Anhui Science and Technology University 2019 (5) 34-39

黄芪甲苷缓解对乙酰氨基酚诱导的肝脏氧化应激损伤

作  者：
刘畅;张乐;朱耀辉;孙克诚;何亚兰;王守开;丁艳梅;胡闪闪;贺焱;李磊
单  位：
安徽科技学院动物科学学院
关键词：
黄芪甲苷;对乙酰氨基酚;急性肝损伤;氧化应激
摘  要：
目的:研究黄芪甲苷(AS-IV)缓解对乙酰氨基酚(APAP)诱导的肝脏氧化应激损伤及其机理。方法:ICR小鼠40只,随机分为4组:空白对照组、APAP模型组(400 mg/kg)、AS-IV低剂量组、AS-IV高剂量组。空白对照组和模型组灌胃0.2 mL的0.5%羧甲基纤维素钠,低剂量组和高剂量组分别给予20 mg/kg和40 mg/kg的AS-IV,1次/天,连续7天后,除空白对照组外,余下3组灌胃400 mg/kg的APAP,灌胃4 h后处死,采集血液和肝脏,检测血清中谷丙转氨酶(ALT),谷草转氨酶(AST)和肝脏中SOD、GSH、MDA,同时采用免疫组织化学法检测各组样本内Nrf2和NF-κB p65的表达情况。结果:模型组血清中ALT、AST活力明显高于空白对照组(P<0.001);与空白组相比,模型组肝脏中GSH含量和SOD活力值显著降低(P<0.01,P<0.001),并且MDA含量显著升高(P<0.001)。与模型组相比, AS-IV(20 mg/kg,40 mg/kg)处理组血清中的ALT、AST活力明显降低(P<0.001),肝脏中GSH的含量与SOD的活力显著升高(P<0.05,P<0.001),MDA的含量显著降低(P<0.001)。免疫组化结果表明,AS-IV可以增加Nrf2的表达水平、降低NF-κB p65的表达水平。结论:AS-IV能够改善对乙酰氨基酚诱导的急性肝损伤,其保护机制与抗氧化应激有关,具有一定的应用前景。
译  名：
Astragaloside IV Ameliorates Acetaminophen-induced Liver Damage by Oxidative Stress
作  者：
LIU Chang;ZHANG Le;ZHU Yaohui;SUN Kecheng;HE Yalan;WANG Shoukai;DING Yanmei;HU Shanshan;HE Yan;LI Lei;College of Animal Science, Anhui Science and Technology University;
关键词：
Astragaloside IV;;Acetaminophen;;Acute liver injury;;Oxidative stress
摘  要：
Objective:To investigate the effects of Astragaloside IV(AS-IV) on the oxidative stress injury induced by acetaminophen(APAP) in liver and its mechanisms.Methods: The model of oxidative stress was established by APAP. Forty ICR mice were randomly divided into 4 groups: blank control group, APAP model group(400 mg/kg), AS-IV low dose group, and AS-IV high dose group. The blank control group and the model group were intragastrically administered with 0.2 mL of 0.5% sodium carboxymethylcellulose, and the low-dose and high-dose groups were given 20 mg/kg and 40 mg/kg of AS-IV, continuous intragastric administration for 7 d. After 7 d, except for the blank control group, the remaining three groups were intragastrically administered with 400 mg/kg of APAP, and sacrificed 4 hours later, collected to blood and liver. Then serum aspartate transaminase(ALT),alanine amiotransferase(AST) and liver superoxide dismutase(SOD), glutathione(GSH), malonaldehyde(MDA) were detected. The expression of Nuclear factor erythroid 2-related factor 2(Nrf2) and NF-κB p65 in each sample was detected by immunohistochemistry. Results: The serum levels of ALT and AST in the model group were both increased significantly than those in the blank control group(P<0.001). Compared with the blank group, the GSH amount and SOD activity in the model group were both decreased significantly(P<0.01, P<0.001), and MDA amount was increased significantly(P<0.001). Compared to the model group, the serum levels of ALT and AST in the AS-IV(20 mg/kg, 40 mg/kg) treatment group were both decreased significantly(P<0.001), and the GSH content and the activity of SOD in the liver were both increased significantly(P<0.05, P<0.001), the content of MDA was decreased significantly(P<0.001). Immunohistochemistry results showed that AS-IV could increase the expression of Nrf2 and decrease the expression of NF-κB p65.Conclusion: AS-IV can improve acetaminophen-induced acute liver injury, and its protective mechanism is related to anti-oxidative stress.

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