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当前位置: 首页 > 文章 > 水貂肠炎病毒双抗体夹心ELISA检测方法的建立 中国兽医科学 2011 (2) 183-187
Position: Home > Articles > Establishment of double antibody sandwich ELISA for detection of mink enteritis virus Chinese Veterinary Science 2011 (2) 183-187

水貂肠炎病毒双抗体夹心ELISA检测方法的建立

作  者：
王建科;程世鹏;易立;杨莘;罗彬;许红丽;闫喜军;武华
单  位：
中国农业科学院特产研究所
关键词：
水貂肠炎病毒;酶联免疫吸附试验;检测;单克隆抗体
摘  要：
以抗水貂肠炎病毒(mink enteritis virus,MEV)单克隆抗体为捕获抗体、兔抗MEV多克隆抗体为检测抗体,建立了MEV双抗体夹心ELISA检测方法。该方法用于MEV抗原的检测。经过试验,单克隆抗体的最适稀释度为1∶20,兔抗MEV多克隆抗体的最适稀释度为1∶3 200。用该ELISA方法分别检测MEV、水貂阿留申病病毒、犬瘟热病毒样品。结果表明,ELISA方法具有良好的特异性。用该ELISA和PCR同时检测158份临床样品,其中ELISA检测40份为阳性,PCR检测44份为阳性,该ELISA的特异性和敏感性分别为95.6%和79.5%,这2种方法的符合率为91.1%。该方法的建立为MEV的检测及水貂病毒性肠炎的流行病学调查提供了工具。
译  名：
Establishment of double antibody sandwich ELISA for detection of mink enteritis virus
作  者：
WANG Jian-ke,CHENG Shi-peng,YI Li,YANG Shen,LUO Bin,XU Hong-li,YAN Xi-jun,WU Hua(Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Jilin 132109,China)
关键词：
mink enteritis virus;ELISA;detection;monoclonal antibody
摘  要：
In order to establish double antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA) for detection of mink enteritis virus(MEV),anti-MEV monoclonal antibody and rabbit anti-MEV polyclonal antibody were used as the capture antibody and detecting antibody,respectively.The optimal dilution of the capture antibody and the detecting antibody capable of detecting MEV antigens were 1∶20 and 1∶3 200 in the check-board titration respectively.Positive samples with MEV,Aleutian mink disease virus and canine distemper virus were examined by the established ELISA respectively.In result,the deve-loped DAS-ELISA had good specificity.A total of 158 samples were tested both by the developed DAS-ELISA and by polymerase chain reaction(PCR).40 of the tested samples were positive by the developed ADS-ELISA and 44 by PCR.The specificity and sensitivity of the developed DAS-ELISA were 95.6% and 79.5%,respectively.The coincidental rate between the developed DAS-ELISA and PCR was 91.1%.These results indicated that the developed DAS-ELISA was suitable for rapid detection and epidemiological investigation of MEV infection in mink.

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