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Position: Home > Articles > Isolation,identification and susceptibility test of the pathogenic Aeromonas veroniifromCarassius auratus gibelio Freshwater Fisheries 2012,42 (5) 22-26

异育银鲫病原维氏气单胞菌的分离鉴定及药敏试验

作  者:
夏飞;梁利国;谢骏
单  位:
农业部淡水渔业和种质资源利用重点实验室;南京农业大学无锡渔业学院;中国水产科学研究院淡水渔业研究中心
关键词:
异育银鲫(Carassius auratus gibelio);维氏气单胞菌(Aeromonas veronii);致病性;药敏试验
摘  要:
从患病的异育银鲫(Carassius auratus gibelio)肝脏中分离得1株优势菌DF-1。将菌DF-1进行人工回感试验,其患病症状同自然发病症状,证明其对鲫有致病性。该菌的形态特征、主要理化特性、16S rRNA序列测定结果及系统发育树的构建均表明其为维氏气单胞菌(Aeromonas veronii)。药敏试验结果表明,该菌对甲哌利福霉素、链霉素、卡那霉素、庆大霉素、四环素、阿洛西林、氟苯尼考等12种药物敏感,对青霉素、苯唑西林、氨苄西林、罗红霉素、克林霉素等15种药物表现出耐药性。
译  名:
Isolation,identification and susceptibility test of the pathogenic Aeromonas veroniifromCarassius auratus gibelio
作  者:
XIA Fei1,2,LIANG Li-guo2,XIE Jun2(1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,Jiangsu,China; 2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China)
关键词:
Carassius auratus gibelio;Aeromonas veronii;pathogenicity;susceptibility test
摘  要:
A dominant bacteria DF-1 was isolated from the liver of sick Carassius auratus gibelio.The challenge test results of using DF-1 to infect C.auratus gibelio displayed that the prevalence of symptoms and spontaneous onset symptoms were the same.It proved that DF-1 was pathogenic to C.auratus gibelio.Morphological,physiological and biochemical characteristics,16S rRNA sequence determination and the construction of phylogenetic tree all indicated that DF-1 was Aeromomas veronii.Susceptibility test showed that helm rifampicin,streptomycin,kanamycin,tetracycline,ampicillin,gentamicin,florfenicol and other 12 kinds of drugs had the most inhibitive effects on the strain.At the same time,it showed resistance to penicillin,oxacillin,ampicillin,amoxicillin,roxithromycin,and other 15 kinds of drugs.

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