中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 草莓斑驳病毒的分子杂交检测 湖南农业大学学报(自然科学版) 2014,40 (3) 273-276
Position: Home > Articles > Detection of Strawberry mottle virus by molecular hybridization Journal of Hunan Agricultural University(Natural Sciences) 2014,40 (3) 273-276

草莓斑驳病毒的分子杂交检测

作  者：
李丽丽;杨洪一
单  位：
黑龙江省林业科学研究所;东北林业大学生命科学学院
关键词：
草莓斑驳病毒;分子杂交技术;cDNA探针
摘  要：
为探索分子杂交技术在草莓斑驳病毒(Strawberry mottle virus,SMoV)检测中的运用,利用CTAB法从草莓叶片中提取总核酸,并以此为模板,利用特异引物扩增SMoV非编码区序列,经克隆、测序获得SMoV非编码区序列。系统进化分析结果显示:本研究中的分离物与GenBank中已报道的分离物的亲缘关系较远,独立形成1个小的进化支。以带有病毒特异片段的质粒为模板,运用PCR技术制备了地高辛标记的cDNA探针。以草莓感染SMoV叶片为试材,利用地高辛标记探针可有效检测草莓叶片中的SMoV,草莓总核酸样品稀释10倍后仍可有效检测。
译  名：
Detection of Strawberry mottle virus by molecular hybridization
作  者：
LI Li-li;YANG Hong-yi;Institute of Forestry Science of Heilongjiang Province;College of Life Science,Northeast Forestry University;
关键词：
Strawberry mottle virus;;molecular hybridization;;cDNA probe
摘  要：
To validate the technique of molecular hybridization on the detection of Strawberry mottle virus(SMoV),total nucleic acid,which extracted from strawberry leaves using CTAB approach,was adopted as a template to be amplified with specific primers in non-coding region of SMoV.And then,sequences of SMoV were obtained after cloning and sequencing phases.The results of phylogenetic analysis indicated that there was a far relative between the isolate in this research and the reported isolates in GenBank,the isolate in the study formed a small unattached branch in phylogenetic tree.Using plasmid DNA with viral specific fragment as a template,a cDNA probe labeled with digoxigenin was prepared by PCR.SMoV could be efficiently detected by the cDNA probe in the test material of strawberry leaves infected by SMoV,as well as in the extreme case with 10 folds dilution solution of total nucleic acid.To our knowledge,this is the first report on the detection of SMoV by cDNA probe.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46