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当前位置: 首页 > 文章 > 山西草莓斑驳病毒cp基因克隆及遗传多样性分析 西北农业学报 2023,32 (12) 2007-2013
Position: Home > Articles > Genetic Diversity of Strawberry Mottle Virus Based On cp Gene Acta Agriculturae Boreali-occidentalis Sinica 2023,32 (12) 2007-2013

山西草莓斑驳病毒cp基因克隆及遗传多样性分析

作  者：
陈伟;李志卫;玉山江·麦麦提;聂园军;李亚娟;赫娟;柴敏;罗永平
单  位：
关键词：
草莓斑驳病毒;cp基因;遗传多样性
摘  要：
旨在研究草莓斑驳病毒(SMoV)在山西省的分布、结构变异及遗传多样性.从山西省7个主要草莓种植区随机采取159份草莓叶片进行RT-PCR病毒检测,结合在线数据库,使用MEGA5构建系统发育树,采用SDTv 1.2软件进行序列相似性分析,运用DnaSP v5.10软件分析cp基因的遗传多样性,采用RDP v.4.31软件检测SMoV中的重组事件.RT-PCR检测后发现,从山西省各地区收集的159份草莓叶片中有65份样品呈阳性,检出率为38.46%.这些阳性样品经过分离、测序、克隆,获得16个SMoV分离物.结合在线的19个SMoV分离物系统进化树分析显示,35个SMoV分离物被分成两组,组1包含28个分离物,均来自中国;组2包含7个分离物,分别来自加拿大、日本和美国.经选择压分析和中性检验,组1和组2 SMoV分离物之间存在显著的遗传差异,且中性检验均为负值,SMoV种群呈扩张状态.序列相似性分析表明,35株分离物的核苷酸同一性为81.34%~100%,氨基酸的一致性为94.77%~100%,呈现出较高的相似性.可见:SMoV存在较高的遗传变异,负选择压力可能是导致SMoV遗传多样性的原因.
译  名：
Genetic Diversity of Strawberry Mottle Virus Based On cp Gene
关键词：
SMoV%cp gene%Genetic diversity
摘  要：
A systematic study of the distribution,structural variation and genetic diversity of SMoV in Shanxi will lay a theoretical foundation for the prevention and control of strawberry virus diseases.In this study,159 strawberry leaves were randomly collected from 7 major strawberry cultivation areas in Shanxi and constructed RT-PCR detection.The cp genes of positive samples were sequenced and ana-lyzed through MEGA5,SDTv 1.2,DnaSP v5.10 and RDP v.4.31,RT-PCR detection showed that 65 samples of the 159 strawberry leaves were positive with a detection rate of 38.46%.The 65 positive samples were isolated,sequenced,and cloned to obtain 16 SMoV isolates.The phylogenetic tree analy-sis,using a dataset that included 19 online SMoV isolates,showed two distinct groups:Group 1,con-sisting of 28 isolates from China,and Group 2,comprising 7 isolates from Canada,Japan,and the Unit-ed States.Selective pressure analysis and neutrality testing indicated significant genetic differences be-tween these groups,with negative neutrality test results suggesting SMoV population expansion.Se-quence similarity analysis displayed that the nucleotide identity range and the consistency range of amino acids was 81.34%-100%and 94.77%-100%respectively,which showed high similarity.This study demonstrated that SMoV has high genetic variation,and the negative selection pressure may be the cause of SMoV genetic diversity,which provides a theoretical guidance for SMoV in Shanxi.

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