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Position: Home > Articles > Cloning of SsPIN2 Gene FromSinningia speciosa and Its Expression in Different Tissues Northern Horticulture 2016 (24) 88-91

大岩桐SsPIN2基因的克隆及组织表达分析

作  者:
徐卫平;刘凤娟;毕春晓;胡鑫;徐全乐;蒋景龙
单  位:
西北农林科技大学生命科学学院;陕西理工大学生物科学与工程学院
关键词:
大岩桐;SsPIN2;基因克隆;表达分析
摘  要:
以大岩桐为试材,采用RT-PCR方法对大岩桐SsPIN2基因进行了克隆,获得898bp的基因序列,经BLAST比对,采用邻接法构建进化树;并利用半定量RT-PCR方法,研究了SsPIN2基因在大岩桐叶、茎尖、根、茎、花瓣和花萼组织的表达情况。结果表明:大岩桐SsPIN2基因与黄瓜CsPIN2、豌豆PsPIN2基因等亲缘关系较近;该基因在上述6种组织中均有表达组织,其中在茎尖、根、茎和花萼的表达量较高,在叶和花瓣中的表达量较低。
译  名:
Cloning of SsPIN2 Gene FromSinningia speciosa and Its Expression in Different Tissues
作  者:
XU Weiping;LIU Fengjuan;BI Chunxiao;HU Xin;XU Quanle;JIANG Jinglong;School of Biological Science and Engineering,Shaanxi Sci-tech University;College of Life Sciences,Northwest A&F University;
关键词:
Sinningia speciosa;;SsPIN2 gene;;gene cloning;;expression analysis
摘  要:
Sinningia speciosa were used as materials,and 898 bp cDNA in length of SsPIN2 gene was isolated.BLAST and phylogenetic trees via neighbor-joining(NJ)method were used to analysis sequence.And semi-quantitative RT-PCR was used to investigate the different expression level of SsPIN2 gene in different tissues of leaf,shoot tips,root,stem,petal and calyx.The results indicated that SsPIN2 shared a high sequence identity with CsPIN2 and PsPIN2.SsPIN2 was expressed in all of the above tissues and relatively higher in shoot tips,root,stem and calyx and relatively lower in leaf and petal.

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