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大岩桐叶片再生体系的建立及卡那霉素敏感性研究

作  者:
尹智慧;宋丽莉;司亮;于典司;郭长虹
单  位:
分子细胞遗传与遗传育种黑龙江省重点实验室
关键词:
大岩桐;组织培养;卡那霉素
摘  要:
以大岩桐(Sinningia speciosa Benth.)幼嫩叶片为外植体,研究了不同激素组合对大岩桐叶片再生体系的影响及抗生素卡那霉素的敏感浓度.结果表明:不同激素组合对不定芽的诱导效果差异显著,叶片不定芽的诱导和增殖的最佳培养基均为MS+1.0mg/L 6-BA+0.1mg/LNAA,生根适宜培养基1/2MS+0.5mg/L NAA,大岩桐叶片对卡那霉素的敏感浓度为200mg/L.
单  位:
Key laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang,College of Life Science and Technology,Harbin Normal University,Harbin,Heilongjiang 150025
关键词:
Sinningia speciosa Benth.%tissue culture%kanamycin
摘  要:
Taking the leaves of Sinningia speciosa Benth.as explants,the concentration of the different hormone combinations of the Sinningia speciosa Benth.leaves regeneration system and the antibiotics sensitive to kanamycin were studied.The results showed that the significantly different effects of different hormone combinations induction of adventitious bud,the optimal medium for leaf induction was MS with 1.0 mg/L 6-BA and 0.1 mg/L NAA,while the 1/2 MS medium with 0.5 mg/L NAA was successful for rapid rooting.The leaves of Sinningia speciosa Benth.with 200 mg/L kanamycin were sensitive.

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