当前位置: 首页 > 文章 > 过量表达tMEK2基因的大岩桐植株对温度胁迫的反应 园艺学报 2012,39 (2) 375-380
Position: Home > Articles > Responses of Sinningia speciosa Overexpressing tMEK2 to Temperature Stresses Acta Horticulturae Sinica 2012,39 (2) 375-380

过量表达tMEK2基因的大岩桐植株对温度胁迫的反应

作  者:
孙姝兰;温秋香;代建丽;邢惕;王小菁
单  位:
卡尔顿大学生物系;华南师范大学生命科学学院
关键词:
大岩桐;tMEK2;过量表达;温度胁迫;恢复
摘  要:
构建番茄中MAPKK基因tMEK2的过量表达载体并转化到大岩桐中,通过PCR和Southernblot鉴定出转基因株系并对其进行抗性分析。结果表明,转基因植株与对照相比叶片变小,叶片的叶绿素含量显著增加,在4℃低温和40℃高温胁迫之后的恢复能力明显比对照增强。
译  名:
Responses of Sinningia speciosa Overexpressing tMEK2 to Temperature Stresses
作  者:
SUN Shu-lan 1,WEN Qiu-xiang 1,DAI Jian-li 1,XING Tim 2,and WANG Xiao-jing 1,*(1Guangdong Key Lab of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou 510631,China;2Department of Biology,Carleton University,Ottawa K1S5B6,Canada)
关键词:
Sinningia speciosa;tMEK2;overexpression;temperature stress;recovery of growth
摘  要:
We constructed a MAPKK gene from tomato,tMEK2 overexpressing construct to transform into Sinningia speciosa.Transgenic plants were identified by PCR and Southern blotting analyses,and their resistance to cold and high temperatures was analyzed.The results showed that the tMEK2 overexpressing plants had smaller leaves,increased contents of chlorophyll in leaves,and better recoveries of plant growth after treatments with cold and high temperatures,compared to control of wild type plants.

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