当前位置: 首页 > 文章 > 食品中黄曲霉毒素B_1 ELISA试剂盒检测方法的建立及验证 湖北农业科学 2016,55 (4) 1008-1012
Position: Home > Articles > Establishment and Validation of Aflatoxin B_1 ELISA Kit Test Method in Foods Hubei Agricultural Sciences 2016,55 (4) 1008-1012

食品中黄曲霉毒素B_1 ELISA试剂盒检测方法的建立及验证

作  者:
谢体波;易重任;陈旭;刘天雷;张磊
单  位:
贵州勤邦食品安全科学技术有限公司
关键词:
黄曲霉毒素B1;ELISA试剂盒;食用油;花生;玉米
摘  要:
建立了黄曲霉毒素B1酶联免疫试剂盒检测的方法,并通过对食用油、花生、谷物中黄曲霉毒素B1测定进行验证。结果表明,在25℃条件下加入50μL标准品溶液、50μL抗体工作液、50μL酶标二抗反应30 min变异性最小;ELISA试剂盒检测灵敏度高,对食用油、谷物、花生样品中黄曲霉毒素B1最低检测限分别为88.82 ng/L和48.51、176.56 ng/kg;平均回收率和变异系数符合相关规定;与高效液相色谱法相比,检测结果一致。
译  名:
Establishment and Validation of Aflatoxin B_1 ELISA Kit Test Method in Foods
作  者:
XIE Ti-bo;YI Zhong-ren;CHEN Xu;LIU Tian-lei;ZHANG Lei;WEI Li-li;LUO Gui-kun;TAO Guang-can;HE Fang-yang;Gui Zhou Kwinbon Food Safety Science-Technology Co.,Ltd.;Beijing Kwinbon Biotechnology Co.,Ltd.;
关键词:
aflatoxin B1;;ELISA kit;;edible oil;;peanut;;maize
摘  要:
This experiment has established the detection method of aflatoxin B1 whit ELISA kit,and based on the determination of aflatoxin B1 in cooking oil, peanuts,corn was validated,the results show that under the condition of 25 ℃,50 μL standard solution,50 μL antibody working liquid, 50 μL enzyme mark two antibody reacted 30 min,variability was minimum;ELISA kit is high detection sensitivity,the aflatoxin B1 minimum detection limit in samples of cooking oil,corn,peanuts,were 88.82 ng / L,48.51 ng / kg,176.56 ng / kg;The average recovery and variation coefficient in accordance with relevant provisions and HPLC.

相似文章

计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊