中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 猪Leptin基因多态性及其与产仔性状的相关性分析 中国畜牧兽医 2017,44 (4) 1122-1127
Position: Home > Articles > The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs China Animal Husbandry & Veterinary Medicine 2017,44 (4) 1122-1127

猪Leptin基因多态性及其与产仔性状的相关性分析

作  者：
朱爱文;周春宝;倪黎纲;韩大勇
单  位：
江苏农牧科技职业学院
关键词：
苏姜猪;姜曲海猪;Leptin基因;PCR-SSCP;产仔性状
摘  要：
试验旨在探究Leptin基因对猪产仔性状的影响,为其新品种持续选育提供遗传学依据。参考GenBank发布的猪Leptin基因DNA序列(登录号:U66254、AF026976)设计3对引物,采用PCR-SSCP技术分析Leptin基因在苏姜猪和姜曲海猪中的单核苷酸多态性(SNP),并用统计学方法分析基因座多态性与产仔性状的相关性。结果发现,Leptin基因在苏姜猪、姜曲海猪群体中均具有多态性,检测到3种基因型:AA、AB和BB;测序结果发现,编码区第3 469位碱基处发生单碱基突变(T3469C),为同义突变。χ2适合性检验显示,2个猪种Leptin基因型分布处于Hardy-Weinberg平衡(P>0.05)。多态信息含量(PIC)分析显示,苏姜猪为中度多态,姜曲海猪为低度多态。Leptin基因型与产仔性状关联分析显示,不同基因型间产仔性状差异不显著(P>0.05),但T3469C位点AB基因型平均产仔数较AA和BB基因型高,初步推断Leptin基因T3469C位点可能是影响猪产仔性状潜在的遗传标记,建议做进一步的研究。
译  名：
The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs
作  者：
ZHU Ai-wen;ZHOU Chun-bao;NI Li-gang;HAN Da-yong;Jiangsu Agri-animal Husbandry Vocation College;
关键词：
Sujiang pigs;;Jiangquhai pigs;;Leptingene;;PCR-SSCP;;litter size trait
摘  要：
The research was aimed to analyze the association of Leptin gene and breed traits on pigs.Three pairs of primers were designed which based on DNA sequence of pig Leptin gene(GenBank accession No.U66254,AF026976),the single nucleotide polymorphisms(SNPs)of Leptin gene were detected by PCR-SSCP in Sujiang and Jiangquhai pigs.The results showed that three genotypes of AA,AB and BB were identified,and the mutation(T3469C)in CDS of Leptin gene was found,but amino acid change weren't happened.The Chi-square test results showed that the Leptin genotype distribution of Sujiang and Jiangquhai pigs was in Hardy-Weinberg equilibrium(P>0.05).The polymorphism information content(PIC)were moderate polymorphism in Sujiang pigs,and low polymorphism in Jiangquhai pigs.The statistic analysis results showed that there were no significant association between the SNP of Leptin genotype and litter size trait(P>0.05).But the average litter size of T3469 Csite AB genotype was higher than AA and BB genotypes,it was concluded that the T3469 Cpolymorphism of Leptin gene might be the genetic marker which potentially affected pig breed traits.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46