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Position: Home > Articles > Establishment of Regeneration System in vitro for Piper methysticum Journal of Anhui Agricultural Sciences 2012,40 (35) 17005-17006+17025

卡瓦胡椒离体再生体系的建立

作  者:
李明芳;郑学勤
单  位:
中国热带农业科学院热带生物技术研究所
关键词:
卡瓦胡椒;离体;再生体系
摘  要:
[目的]建立卡瓦胡椒(Piper methysticum)的离体再生体系。[方法]以卡瓦胡椒的带芽嫩茎和嫩叶作为外植体,通过在培养基中添加不同的激素组合,来筛选最佳再生培养基。[结果]卡瓦胡椒的丛芽诱导培养基为MS+4 mg/L 6-BA+0.5 mg/L GA3+0.65%琼脂+3%蔗糖+0.012 5%PVP,最佳芽伸长根生长培养基为1/2MS+5 nmol/L JA+0.65%琼脂+3%蔗糖。[结论]为卡瓦胡椒的工业化生产提供了理论指导。
译  名:
Establishment of Regeneration System in vitro for Piper methysticum
作  者:
LI Ming-fang et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101)
关键词:
Piper methysticum;in vitro;Regeneration system
摘  要:
[Objective] This study aimed to establish in vitro regeneration system for Piper methysticum.[Method] With tender kava stems and leaves with bud as explants,different hormone combinations were added into basic medium,to screen the optimal regeneration medium.[Result] The optimal induction medium for multiple buds of kava was MS + 4 mg/L 6-BA + 0.5 mg/L GA3+0.65% agar + 3% sucrose + 0.012 5% PVP;the optimal regeneration medium for kava plantlets was 1/2MS +5 nmol/L JA +0.65% agar +3% sucrose.[Conclusion] This study provides theoretical guidance for the enlargement of industrial production of kava.

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