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Position: Home > Articles > Studies on the AFLP Molecular Markers of Kava and Piper nigrum Journal of Henan Agricultural Sciences 2007,36 (7) 82-86

卡瓦胡椒及胡椒的荧光AFLP分子标记研究

作  者:
施江;辛莉;杨彦;郑学勤
单  位:
北京鼎国生物技术有限责任公司;河南科技大学农学院;热带作物生物技术国家重点实验室
关键词:
卡瓦胡椒;胡椒;扩增片段长度多态性;亲缘关系
摘  要:
对6份卡瓦胡椒材料、23份栽培胡椒和野生胡椒材料、1份不同属的草胡椒材料、1份不同科的普通烟材料,共计31份试验材料,进行荧光AFLP分子标记,研究卡瓦胡椒与胡椒及其他近缘野生种的亲缘关系。结果表明,在相似系数0.52处将31份种质划分为5个类群,第1个类群有普通烟,第2个类群有草胡椒,第3个类群是卡瓦胡椒,第4个类群有胡椒,第5个类群有山胡椒、蒌叶和蒟蒌。说明卡瓦胡椒与胡椒及其他近缘野生种的亲缘关系有一定距离,为卡瓦胡椒真伪性的分子鉴定和构建卡瓦胡椒的指纹图谱提供了基础性的工作。
译  名:
Studies on the AFLP Molecular Markers of Kava and Piper nigrum
作  者:
SHI Jiang1,XIN Li1,YANG Yan2,ZHENG Xue-qin3(1.College of Agronomy,Henan University of Science and Technology,Luoyang 471003,China;2.Beijing Dingguo Biotechnology CO.,LTD,Beijing 100088,China;3.State Key Laboratory of Tropitcal Crop Biotechnology,Haikou 571101,China)
关键词:
Kava;Piper nigrum;Amplified fragment length polymorphism;Genetic relationship
摘  要:
The genetic relationship between Kava and Piper nigrum has been studied by the AFLP molecular markers.31 test materials were used in the paper,including 6 Kava genotypes,23 cultivated and wild peppers,1 Peperomia pellucida kunth and 1 tobacco.Based on the fluorescence-labeled AFLP,the 31 germplasms were devided into 5 different groups at 0.52 of the genetic similarity coefficient.The result showed that the genetic distance between Kava and other Piper germplasms was far,and the fluorescence-labeled AFLP was applicable to the identification and DNA fingerprint construction of Kava.

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