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Position: Home > Articles > Amplification of a Kava Specific SCAR Molecular Marker Chinese Journal of Tropical Crops 2007,28 (2) 50-53

1个与卡瓦胡椒特异的SCAR标记的获得

作  者:
施江;辛莉;谭琳;郑学勤
单  位:
河南科技大学农学院;热带作物生物技术国家重点实验室
关键词:
卡瓦胡椒;胡椒;RAPD;SCAR
摘  要:
采用6份卡瓦胡椒材料、21份栽培胡椒和野生胡椒材料、1份不同属的草胡椒材料共计28份试验材料,在对它们进行了RAPD研究的基础上,进行了SCAR分子标记研究。设计1对卡瓦胡椒特异SCAR引物P8.1和P8.2,用这对特异引物对本次试验的28份材料进行PCR扩增,结果只有6份卡瓦胡椒材料扩增出了预期大小355bp的特异带,其它材料均无任何扩增。这说明引物P8.1和P8.2为卡瓦胡椒特异SCAR引物,这对卡瓦胡椒种质资源的鉴定有一定帮助。
译  名:
Amplification of a Kava Specific SCAR Molecular Marker
作  者:
Shi Jiang1 Xin Li1 Tan Lin2 Zheng Xueqin2 1 Agriculture College,Henan University of Science & Technology Luoyang Henan 471003 2 State Key Biotechnology Laboratory for Tropical Crops,Chinese Academy of Tropical Agricultural Sciences Haikou Hainan 571101 China
关键词:
Piper methysticum Piper nigrum Peperomia pellucida Kunth random amplified polymorphic DNA (RAPD) sequence-characterized amplified region(SCAR)
摘  要:
In this experiment 6 accessions of kava(Piper methysticum),21 accessions of cultivated pepper and wild pepper,1 accession of Peperomia pellucida Kunth(28 accessions in all)were analysed with RAPD and then SCAR molecular marker.A pair of kava specific SCAR primers of P8.1 and P8.2 were designed and used to amplify 28 accessions by PCR.A specific band of an expected size of 355bp was amplified only from 6 kava accessions.This showed that P8.1 and P8.2 are two primers specific to kava and can be used for molecular identification of kava germplasm.

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