当前位置: 首页 > 文章 > 药用植物草麻黄细胞的悬浮培养 安徽农业科学 2012,40 (3) 1419-1420
Position: Home > Articles > Cell Suspension Culture of Medicinal Plant Ephedrae sinica Stapf Journal of Anhui Agricultural Sciences 2012,40 (3) 1419-1420

药用植物草麻黄细胞的悬浮培养

作  者:
张红梅;王明艳;蔡宝宏;信佳言
单  位:
唐山市第一中学生物教研室;河北联合大学生命科学学院
关键词:
草麻黄;愈伤组织;悬浮培养
摘  要:
[目的]对草麻黄的子叶诱导出的愈伤组织,进行悬浮培养。[方法]采用组织培养的方法进行了愈伤组织诱导、愈伤组织继代和悬浮培养研究。[结果]MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA为诱导子叶形成愈伤组织的理想培养基;MS+1.5 mg/L 2,4-D+1.5 mg/L6-BA是愈伤组织的适宜继代培养基;2.0 mg/L 2,4-D+1.5 mg/L 6-BA+300 mg/L水解酪蛋白(CH)是愈伤组织悬浮培养的适宜培养基,愈伤组织干重增加量为0.80 g。[结论]初步选择出草麻黄愈伤组织细胞的悬浮培养条件,为麻黄细胞扩大培养及有效成分提取奠定基础。
译  名:
Cell Suspension Culture of Medicinal Plant Ephedrae sinica Stapf
作  者:
ZHANG Hong-mei et al (College of Life Science,Hebei United University,Tangshan,Hebei 063000)
关键词:
Ephedrae sinica Stapf;Callus;Suspension culture
摘  要:
[Objective] Ephedra callus induced with Ephedra explant were cultured in suspension culture.[Method] The conditions needed in callus induction,callus generation and suspension culture of Ephedrae sinica Stapf cotyledon were studied.[Result] The results showed that the best medium for callus induction was MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA.The best media of callus generation was MS+1.5 mg/L 2,4-D+1.5 mg/L 6-BA.The best media of suspension culture was 2.0 mg/L 2,4-D+1.5 mg/L 6-BA+CH 300 mg/L,increased amount of dry weight was 0.80 g.[Conclusion] The condition of Ephedrae sinica Stapf suspension culture was preliminary established,which will lay a foundation for the expanding culture and extracting active ingredient of Ephedrae sinica Stapf cell.

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