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当前位置: 首页 > 文章 > 影响猪卵母细胞玻璃化冷冻保存效果的因素 畜牧与饲料科学 2010,31 (3) 99-102
Position: Home > Articles > Effects of Several Factors on Cryopreservation of Porcine Oocytes by Vitrification Animal Husbandry and Feed Science 2010,31 (3) 99-102

影响猪卵母细胞玻璃化冷冻保存效果的因素

作  者：
Xuguang Li
单  位：
College of Animal Science and Technology, AnHui Agricultural University, Hefei 230036, Chin
关键词：
cryopreservation;porcine oocytes;oocytes;vitrification;effects of;effective method
摘  要：
对卵母细胞进行冷冻保存,可以使卵源不再受到时间和空间的限制,并能为体外受精、核移植和转基因等胚胎工程技术的研究提供充足的原材料。猪卵母细胞中富含脂滴,且对低温十分敏感,因此,猪卵母细胞冷冻保存的研究进展相对较慢。玻璃化冷冻是有效的卵母细胞冷冻方法之一。综述了卵母细胞发育阶段、冷冻载体和冷冻保护剂等因素对猪卵母细胞玻璃化冷冻保存效果的影响。
译  名：
Effects of Several Factors on Cryopreservation of Porcine Oocytes by Vitrification
作  者：
DAI Zhi-jun1,2,TAO Yong2,LIU Xu-guang2 (1.Reproductive Medical Center,Hefei Maternal and Child Health Hospital,Hefei 230001,China;2.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China)
关键词：
pigs;oocytes;vitrification;cryopreservation
摘  要：
Cryopreservation can make the use of oocytes not constrained by time and space,and the cryopreserved oocytes can be used as materials for in vitro fertilization,nuclear transfer,transgene and other embryo engineering technology.Porcine oocytes are highly sensitive to lower temperatures due to rich lipid droplets in ooplasm.As a result,researches about cryopreservation of porcine oocytes get less progress.Vitrification is one of the effective methods for cryopreservation of oocytes.This paper discusses the effects of developmental stage of oocytes,cryopreservation carriers and cryoprotectant on cryopreservation of porcine oocytes by vitrification.

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