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LDL对绵羊精子冷冻保存效果的研究

作  者:
Zheng Yun-shen
单  位:
College of Animal Science and Medecine,Inner Mongolia Agricultural University,Huhhot 010018,China
关键词:
sheep;ldl;egg yolk;cryopreservation;freezing;fertility tes
摘  要:
绵羊冷冻精液的受精率低于自然交配,其原因之一可能是在冷冻过程中精子质膜损伤严重造成的。该研究以内蒙古细毛羊为试验动物,在稀释液中以低密度脂蛋白(LDL)代替卵黄。分析了冷冻过程中对精子活率、顶体完整率和质膜完整率的影响。筛选出了最优的LDL稀释液配方,以提高绵羊精液质量,减少精子的损伤,为提高绵羊精子冷冻保存后的受精率提供理论依据。
译  名:
Effects of LDL on Cryopreservation of Sheep Spermatozoa
作  者:
HONG Wei,NI Li-ping,TIAN Dong-hai,LU Yong-yue,DENG Chang-sheng,LI Jin-quan,ZHANG Xiao-yu,ZHENG Yun-sheng(College of Animal Science and Medecine,Inner Mongolia Agricultural University,Huhhot 010018,China)
关键词:
sheep semen;LDL;egg yolk;cryopreservation;sperm quality
摘  要:
The fertility test showed that pregnant rate of AI with frozen semen were significantly lower than that of natural mating. One of the reasons might be the sperm plasma membrane was serious injured during freezing. Therefore,the paper studied on sheep spermatozoa during freezing which was protected with egg yolk(EY) and local fine wool sheep was used as the experimental animals. Then low density lipoprotein(LDL) was used in diluents instead of egg yolk to study on the effect of LDL on sperm motility,acrosome intact rate and plasma membrane integrity. The optimal LDL dilution formula was screened to improve sheep semen quality,reduce the damage sperm,and provide theoretical basis for improving fertility of ram sperm after cryopreservation.

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